D/swine/Italy/173287-4/2016


EVAg

Originator
:
https://www.izsler.it/
Produced by
: IZSLER
Shipping From
: Brescia - IT

Product Description

Ref-SKU:
026V-04378
The virus has been isolated from swine and propagated on HRT cell line
Product Risk Group: 

Storage conditions: 
Freeze Dried
Sequencing: 
Complete genome
Infectivity: 
Infectivity tested and quantified
Mycoplasmic content: 
Not tested

Unit definition: 
1 mL

In stock

550,00 €
(Cost per access for Academics)

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EVAg

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virus

GMO: 
No
Biosafety restrictions: 
Human Risk Group 2, Animal Risk Group 2
Virus host type: 
Previous Name or Taxonomy: 
Infectivity Test: 
HA test
Viral titer: 
HA titer >128
Production cell line: 
Genbank reference: 
Virus Cultivability: 
Passage: 
2 X HRT-18
Identification technique: 
PCR, sequencing
Technical recommendation: 
PCR, sequencing
Shipping conditions: 
IATA Classification: 

Information about the collection of the virus

Recombinant product: 
No
Biological material origin: 
Natural origin
Collection date: 
Tuesday, 21 June, 2016
Country of collection: 
Suspected epidemiological origin : 
Isolation host: 
Isolation technique: 
Propagation on HRT-18 cell line
Isolation conditions: 
HRT-18 cells were cultured in 25 cm2 tissue culture flask in MEM and 10% foetal calf serum. When the cells formed a monolayer, usually after 24 h /48 h, the medium was removed and cells were washed three times with saline solution. Each sample was inoculated onto HRT-18 cells (1ml) followed by 1ml of MEM . The inoculum was allowed to adsorb at 37 °C for 45 min in presence of 5% CO2 and then 8 ml of MEM was added.