EVAg

Training in reverse genetics method (ISA method), recently developed to reconstitute an infectious Flavivirus or Alphavirus under BSL-3 conditions. The training comprises DNA fragments amplification by PCR; Transfection of the purified products into permissive cells; Viral culture; Molecular detection

Freeze-dried Tyuleniy virus, strain UVE/TYUV/UNK/XX/3-ARCH-Ori, complete genome sequence

Pothos latent virus, infectious isolate

12 freeze dryed inactivated samples in screw cap tubes

Viral RNA extracted from infected cell culture lysate.

Olive latent virus-1 from Citrus

Herpes simplex virus Plasmid for real time PCR. The target region of a specific real time assay was amplified and cloned.

Invitro transcript for Hepatitis C virus genomic 5`ncr region assays. The target region of a specific real time assay was amplified and cloned.

HCV X-tail Plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Dengue virus serotype 2 plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Invitro transcript for Hepatitis C virus genomic x-tail region assays.

Dengue virus serotype 3 plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Dengue virus serotype 4 plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Dengue virus serotype 1 plasmid for invitro transcription - Target region of a specific real time assay was amplified and cloned.

Dengue virus serotype 1 RNA extracted from infected cell culture lysate.

West Nile virus plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

in vitro transcribed RNA for a specific Yellow fever virus real time PCR

Yellow fever virus plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

In vitro transcribed RNA for a specific JEV (Japanese encephalitis virus) real time PCR, here: NS2A (Ref: Article Source: Development of an improved RT-qPCR Assay for detection of Japanese encephalitis virus (JEV) RNA including a systematic review and comprehensive comparison with published methods Bharucha T, Sengvilaipaseuth O, Vongsouvath M, Vongsouvath M, Davong V, et al. (2018) Development of an improved RT-qPCR Assay for detection of Japanese encephalitis virus (JEV) RNA including a systematic review and comprehensive comparison with published methods. PLOS ONE 13(3): e0194412.

In vitro transcribed RNA (positive control) for a Usutu virus real time PCR. (Ref: J Virol Methods. 2014 Mar;197:51-4. doi: 10.1016/j.jviromet.2013.08.039. Epub 2013 Sep 13, "Development of a Usutu virus specific real-time reverse transcription PCR assay based on sequenced strains from Africa and Europe." Nikolay B1, Weidmann M2, Dupressoir A3, Faye O4, Boye CS5, Diallo M6, Sall AA7.)

Stratford virus C338

ZIka virus, laboratory strain.

Zika virus isolated from semen sample. Early passage.

Chikungunya virus, Guatemala isolate, isolated from UK patient

Tomato aspermy virus from chrysanthemum