The full-length genome of TYLCSV (1.8 copies) is cloned in the binary plasmid vector pBin19, allowing transformation into Agrobacterium and infection of plants
Unit: 1 microgram, allowing infection of plants following transformation into Agrobacterium
Carnation Italian ringspot virus full-length cDNA cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNA
Unit: 2 micrograms plasmid DNA, allowing for the inoculation of six host plants (Nicotiana benthamiana) after T7-driven transcription
The full-length genome of CpCDV (1.8 copies) is cloned in the binary plasmid vector pBin19, allowing transformation into Agrobacterium and infection of plants
Unit: 1 microgram, allowing infection of plants following transformation into Agrobacterium