EVAg

The full-length genome of TYLCV (1.7 copies) is cloned in the binary plasmid vector pCambia 0380, allowing transformation into Agrobacterium and infection of plants.

The full-length genome of TYLCV-Mld (2 copies in tandem) is cloned in the binary plasmid vector pCambia 2300 allowing transformation into Agrobacterium and infection of plants

The full-length genome of PYMV-A (1.44 copies) is cloned in the binary plasmid vector pCambia 0380 allowing transformation into Agrobacterium and infection of plants

The full-length genome of PYMV-B component (1.54 copies) is cloned in the binary plasmid vector pCambia 0380, allowing transformation into Agrobacterium and infection of plants

The full-length genome of Tomato leaf curl Comoros virus(1.2 copies) is cloned in the binary plasmid vector pCambia 2300, allowing transformation into Agrobacterium and infection of plants

In this two-week course, to be held in the CNR-IPSP laboratories in Torino (Italy), the basic principles of geminivirus typing and molecular characterization will be addressed by a combination of lectures, demonstration and hands-on training. Topics include: sample preparation, nucleic acid extraction, rolling-circle amplification, RFLP analysis, cloning, sequencing, phylogenetic analysis.

In this five-day course, to be held in the CNR-IPSP laboratories in Torino (Italy), the basic principles of geminivirus diagnosis will be addressed by a combination of lectures, demonstration and hands-on training. Topics include: tissue-printing hybridization, dot-blot hybridization, preparation of non-radioactive probes, rapid sample extraction. After this course participants should have sufficient knowledge to introduce routine diagnostics of geminiviruses in their own laboratory.