EVAg

In this two-week course, to be held in the CNR-IPSP laboratories in Torino (Italy), the basic principles of geminivirus typing and molecular characterization will be addressed by a combination of lectures, demonstration and hands-on training. Topics include: sample preparation, nucleic acid extraction, rolling-circle amplification, RFLP analysis, cloning, sequencing, phylogenetic analysis. Participants may bring their own samples following agreement with the organizers. After this course participants should have sufficient knowledge to introduce molecular characterization of geminiviruses (and other small circular DNAs) in their own laboratory.