- Tagged as
- - Derived product
- - Derived product
- - Service
Produced by
: DSMZ Shipping From
: Braunschweig - DE
Product Description
Ref-SKU:
027S-04177 In an outbreak situation in crops, it is necessary to obtain quickly a good overview of the viruses that may be involved. Only in this way can the right measures be taken to safeguard crop production immediately but also in the long term. High-throughput sequencing (HTS) and bioinformatics analysis of samples offer a global analysis of the phytosanitary status of plants and the rapid and precise identification of pathogens. This service package offers the individual analysis of three plant samples by HTS to ensure a reliable detection of the potentially involved viruses in an outbreak situation.
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Virus host type:
Can it produce GMO:
No
Comments (5)
Diversity and distribution of sweet potato viruses in Zambia: new knowledge and tools to support food production and seed systems
Sweet potato (Ipomoea batatas, family Convolvulaceae) is the third most important root and tuber crop across the globe due to its ability to both withstand extreme hard climatic conditions and produce edible tuberous roots that can perform well in poor soils. The production of this vegetatively propagated crop is compromised severely by the impact of viruses. More than 30 viruses have been reported in sweet potato. The sweet potato virus disease complex (SPVD) is the most important viral disease and it is caused by the synergistic coinfection of Sweet potato chlorotic stunt virus (SPCSV; genus Crinivirus) and Sweet potato feathery mottle virus (SPFMV; genus Potyvirus). In Zambia, sweet potato is a food security crop and grown within smallholder agro-ecosystems country-wide. Most farmers in Zambia usually exchange and recycle planting material that is of low quality and highly susceptible to viral diseases leading to the propagation of viruses and impacting the sustainability of a sweet potato seed system in the country. To date information about the distribution and diversity of sweet potato viruses in Zambia is scarce. This study will close this gap and provide data on contemporary virus species and variants in sweet potato in the country. The proposal aims to investigate the prevalence and distribution of sweet potato viruses in major sweet potato production regions in Zambia.
Sweet potato samples (n = 500) will be collected from the main producing regions in Zambia. These samples will be initially screened using established nitrocellulose membrane - enzyme-linked immunosorbent assay (NCM-ELISA) protocols (Fuentes et al., 2019. HQU ‐ Sweet potato NCM‐ELISA Virus Detection CIP‐SOP021, v.1.1 11 pps). Selected samples will then be tested by high-throughput sequencing (HTS) to determine the genetic diversity and distribution of sweet potato viruses in Zambia. In this context, we would like to take advantage of the European Virus Archive-Global (EAVg) project and to apply to the EVAg-TNA initiative in order to investigate the presence of known and novel viruses in the samples. We propose the analysis of three pools. This opportunity will allow us to for the first time determine the diseases that are unidentified and which potentially could be associated with disease outbreaks in sweetpotato production in Zambia and whose information will aid sweetpotato yield reduction and consequently safeguard food production. My institution does not have the resources such as upstream sequencing infrastructure and fianacial resources to undertake such a sequencing initiative. The new HTS data will allow the design of novel and specific diagnostic tests for a rapid on-site detection of viruses in sweet potato crops. There will be an improved knowledge of the diversity and distribution map of the sweet potato viruses and optimized diagnostics protocols. The outputs will be disseminated to extension services and farmers to raise awareness and engagement contributing to more efficient control strategies.
Publications:
Mulema J, Phiri S, Bbebe N, Chandipo R, Chijikwa M, Chimutingiza H, Kachapulula P, Kankuma Mwanda F, Matimelo M, Mazimba-Sikazwe E, Mfune S, Mkulama M, Moonga M, Mphande W, Mufwaya M, Mulenga R, Mweemba B, Ndalamei Mabote D, Nkunika P, Nthenga I, Tembo M, Chowa J, Odunga S, Opisa S, Kasoma C, Charles L, Makale F, Rwomushana I, Phiri NA. (2024). Rapid risk assessment of plant pathogenic bacteria and protists likely to threaten agriculture, biodiversity and forestry in Zambia. NeoBiota 91: 145–178. https://doi.org/10.3897/neobiota.91.113801
Tembo, M., Lubungu, M., Singogo, F. K., Mwanza, M., Onyango, M., Sakala, P., Selvaggio, M. P., Berhane, E. (2023). Maize and groundnut crop production among rural households in Zambia: implications in the management of aflatoxins. Food Control 154:109964. doi: 10.1016/j.foodcont.2023.109964
Chikoti, P. C and Tembo, M. (2022). Expansion and impact of cassavabrown streak and cassava mosaicdiseases in Africa: A review. Frontiers in Sustainable Food Systems 6:1076364. doi: 10.3389/fsufs.2022.1076364
Szyniszewska, A. M., Chikoti, P. C., Tembo, M., Mulenga, R., Gilligan, C. A., Bosch, F., McQuaid, C. F. (2021). Smallholder cassava planting material movement andgrower behavior in Zambia: implications for the management of cassava virusdiseases. Phytopathology 111, 1952–1962. doi: 10.1094/PHYTO-06-20-0215-R
Tembo, M., Mwansa, K., Kambukwe, K., Ndeke, V., Ng’uni, D., Chibwe, L., Magorokosho, C., & Suresh, L. M. (2021). Screening of maize germplasm for resistance to maize lethal necrosis disease in Zambia. African Journal of Biotechnology , 20(1), 25-32. https://doi.org/10.5897/AJB2020.17255
Tembo, M., Adediji, A.O., Bouvaine, S., Seal, S.E. & Silva, G. (2020). A quick and sensitive diagnostic tool for detection of Maize streak virus. Sci Rep 10, 19633. https://doi.org/10.1038/s41598-020-76612-2
Chiza Chikoti P, Tembo M, Peter Legg J, Rufini Shirima R, Mugerwa H, Sseruwagi P. (2020). Genetic Diversity of Mitochondrial DNA of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) Associated with Cassava and the Occurrence of Cassava Mosaic Disease in Zambia. Insects. 2020; 11(11):761. https://doi.org/10.3390/insects11110761
Chikoti, P.C., Mulenga, R.M., Tembo, M. & Sseruwagi, p. (2019). Cassava mosaic disease: a review of a threat to cassava production in Zambia. Journal of Plant Pathology 101, 467–477 (2019). https://doi.org/10.1007/s42161-019-00255-0
Mukanga, M., Tembo, M. & Mudenda, M. (2019). Incidence of Mycotoxin Contamination along the Maize Value Chain in Lusaka Province of Zambia. International Journal of Agriculture and Biological Sciences, 3(4), 69–77. https://doi.org/10.5281/zenodo.3484487
Graziosi I, Tembo M, Kuate J, Muchugi A. (2020). Pests and diseases of trees in Africa: a growing continental emergency. Plants People Planet. 2020;2:14–28. https://doi.org/10.1002/ppp3.31.
Tembo M, Mataa M, Legg J, Chikoti P.C, Ntawuruhunga P. (2017). Cassava mosaic disease: Incidence and yield performance of cassava cultivars in Zambia. Journal of Plant Pathology. 99(3):1-28. DOI: https://doi.org/10.4454/jpp.v99i3.3955
Chikoti, P. C., Tembo, M., Chisola, M., Ntawuruhungu, P., and Ndunguru, J. (2015). Status of cassava mosaic disease and whitefly population in Zambia. African Journal of Biotechnology. 14, 2539–2546. doi: 10.5897/AJB2015.14757
Chikoti, P. C., Tembo, M., Chisola, M., Mwaba, H., Mulenga, R., Sichilima, S. and Matimelo, M. (2018). In: Sseruwagi, P, Tairo, F, Ndunguru. J. The Cassava Diagnostics Project (CDP): A review of 10 years of research 2008–2018. Dar es Salaam, Tanzania: Tanzania Agricultural Research Institute (TARI)-Mikocheni; 2018.
Viral plant diseases are among the most important causes of yield and income losses for populations in developing countries. Indeed, phytoviruses have a devastating impact on the poorest communities whose livelihoods depend on agriculture.
Against a backdrop of recurrent socio-political and humanitarian crises over several decades, the Central African Republic (CAR) is a country where everything is a priority. Its human development indices are among the lowest in the world. Insufficient agricultural production due to security instability has led to food insecurity. The direct consequences are food insecurity, hunger and malnutrition, which affects more than half of the Central African population.
Vegetable crops in general, and sweet potatoes in particular, are among the crops that make an effective contribution to the fight against food insecurity, hunger and poverty in CAR, given their shorter cycles (2 to 5 months) and their agronomic and nutritional qualities. Unfortunately, these crops are highly affected by numerous viral diseases. In recent years, various symptoms have been observed on market garden crops and sweet potatoes in urban and peri-urban areas of Bangui. The real causes of these observations are little or unknown to growers and researchers alike, due to the lack of data on viral diseases affecting these crops in the Central African Republic.
Indeed, in CAR, scientific research in the field of crop protection in general, and plant virology in particular, is barely explored. Little is known about viral diseases of cultivated plants in the Central African Republic, due to the lack of equipment and resources in research structures.
However, in a highly globalized and interconnected world, with the implications of climate change, viral plant diseases are becoming increasingly widespread, favoring the appearance of emerging viral diseases, which are difficult to anticipate due to their new and unpredictable nature. And yet, to implement effective control strategies, it´s imperative to expand our knowledge on the viruses present in our crops and on their genetic diversity.
It is in this context that this project proposes to carry out a broader inventory of the phytovirome of okra, tomato and sweet potato crops, using new metagenomic approaches. In particular, high-throughput sequencing will enable us to identify all the viral genomes present in the samples analyzed. Molecular characterization of these viral genomes will lead to the identification of viral species associated with viral diseases of these crops in CAR. These data will enable the development of molecular diagnostic and monitoring tools for viral crop diseases.
We would like to take advantage of the European Virus Archive-Global (EAVg) project and apply for the EVAg-TNA. This opportunity will allow us to conduct for the first time in our country global surveys on pathogens of primary crops, which have been so far hampered due to the availability of significant infrastructures and resources to support such projects.
« Detection and molecular characterization of viruses infecting okra, sweet potato and tomato in the Central African Republic using next-generation sequencing (NGS) ».
Viral plant diseases are among the most important causes of yield and income losses for populations in developing countries. Indeed, phytoviruses have a devastating impact on the poorest communities whose livelihoods depend on agriculture.
Against a backdrop of recurrent socio-political and humanitarian crises over several decades, the Central African Republic (CAR) is a country where everything is a priority. Its human development indices are among the lowest in the world. Insufficient agricultural production due to security instability has led to food insecurity. The direct consequences are food insecurity, hunger and malnutrition, which affects more than half of the Central African population.
Vegetable crops in general, and sweet potatoes in particular, are among the crops that make an effective contribution to the fight against food insecurity, hunger and poverty in CAR, given their shorter cycles (2 to 5 months) and their agronomic and nutritional qualities. Unfortunately, these crops are highly affected by numerous viral diseases. In recent years, various symptoms have been observed on market garden crops and sweet potatoes in urban and peri-urban areas of Bangui. The real causes of these observations are little or unknown to growers and researchers alike, due to the lack of data on viral diseases affecting these crops in the Central African Republic.
Indeed, in CAR, scientific research in the field of crop protection in general, and plant virology in particular, is barely explored. Little is known about viral diseases of cultivated plants in the Central African Republic, due to the lack of equipment and resources in research structures.
However, in a highly globalized and interconnected world, with the implications of climate change, viral plant diseases are becoming increasingly widespread, favoring the appearance of emerging viral diseases, which are difficult to anticipate due to their new and unpredictable nature. And yet, to implement effective control strategies, it´s imperative to expand our knowledge on the viruses present in our crops and on their genetic diversity.
It is in this context that this project proposes to carry out a broader inventory of the phytovirome of okra, tomato and sweet potato crops, using new metagenomic approaches. In particular, high-throughput sequencing will enable us to identify all the viral genomes present in the samples analyzed. Molecular characterization of these viral genomes will lead to the identification of viral species associated with viral diseases of these crops in CAR. These data will enable the development of molecular diagnostic and monitoring tools for viral crop diseases.
We would like to take advantage of the European Virus Archive-Global (EAVg) project and apply for the EVAg-TNA. This opportunity will allow us to conduct for the first time in our country global surveys on pathogens of primary crops, which have been so far hampered due to the availability of significant infrastructures and resources to support such projects.
Viral diseases are one of the most important causes of yield losses in agricultural production, having a socio-economic impact on farmers in developing countries.
Control strategies to reduce the effects of these diseases depend on a better understanding of the viral species/strains involved. Identifying all the viruses infecting cultivated plants is proving difficult, given the new viruses that may be associated with the symptoms observed. This is why the opportunity offered by the European Virus Archive-Global (EAVg) project, with its high-throughput sequencing, is so useful.
Viral diseases are one of the most important causes of yield losses in agricultural production, having a socio-economic impact on farmers in developing countries.
Control strategies to reduce the effects of these diseases depend on a better understanding of the viral species/strains involved. Identifying all the viruses infecting cultivated plants is proving difficult, given the new viruses that may be associated with the symptoms observed. This is why the opportunity offered by the European Virus Archive-Global (EAVg) project, with its high-throughput sequencing, is so useful.