EVAg

Virus whole genome sequencing

Course instruction will be conducted in the high containment facility at ACDP, Geelong, Victoria, Australia. Participants will receive a 5-day intensive course that includes interactive theoretical and practical experiences. Topics addressed will include introduction to BSL4 suited laboratories, regulatory frameworks, operational oversight, engineering controls and maintenance considerations.

An in-depth theoretical and practical application of biosafety, biocontainment and biosecurity under the Australia/New Zealand standards and Australian regulatory framework for Biosecurity Containment and Physical Containment level 3 and 4 laboratory and animal facilities.

During visit of the facility examples for comprehensive detection and measures to control quarantine pathogens (viruses, bacteria, fungi, nematodes) will be presented and the associated quality management will be demonstrated. Furthermore time for mutual scientific exchange and discussions on the control of known and emerging viruses is given. The number of applicants is limited to one to two persons per visiting period.

Human Immunodeficiency Virus (HIV) drug resistance remains a significant health issue that challenges treatment and prevention. The objective of this 3-week course is to learn bioinformatics techniques for the analysis of next-generation HIV sequencing results. This covers both HIV-1 and HIV-2. Teaching objectives include data curation, alignment, phylogenetics for typing, identification of drug resistance mutations, production of a resistance score, and interpretation. This course does not include genotyping itself, only sequence analysis and interpretation, as described above.

In this five-day course, to be held in the CNR-IPSP laboratories in Torino (Italy), the basic principles of geminivirus diagnosis will be addressed by a combination of lectures, demonstration and hands-on training. Topics include: tissue-printing hybridization, dot-blot hybridization, preparation of non-radioactive probes, rapid sample extraction. After this course participants should have sufficient knowledge to introduce routine diagnostics of geminiviruses in their own laboratory.

Training in Coronavirus reverse genetics to adapt novel techniques (ISA method) to large RNA viruses and compare different technologies under BSL-3 conditions

SARS-CoV-2 diagnostic training, including differential diagnostics of human Coronaviruses and other respiratory pathogens, incl. BSL-2 and BLS-3 conditions.

In this 3-day course the basic principles of the protein microarray will be addressed by a combination of lectures, demonstration and hands-on training. The protein microarray technique can be used for HA antibody profiling for group level assessment of exposures to influenza viruses in small sample volumes from humans and animals (birds, horses, pigs, bats, carnivores a.o.). The training encompasses wet lab training but also the analysis and interpretation of assay results. Special attention will be given to specific issues of Influenza and flavivirus cross reactivity.

Diagnostic training in zooonotic viruses under BSL-2/BSL-3 conditions at the Institute of Virology - Charité Universitätsmedizin Berlin, Germany.

Comparative arbovirus diagnostics training, including molecular and serologic techniques under BSL-2 and BSL-3 conditions

Kidney cells (epithelial)/ fibroblast–like, origin: Chlorocebus sabaeus (African green monkey); CRISPR/Cas9-engineered IFN-Lambda deficient phenotype