EVAg

Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 4x24 rxns) for real time RT-PCR of SARS-CoV-2 (2019-nCoV) as of Jan 30, 2020, available from https://www.mdpi.com/1999-4915/12/2/159/pdf. Primers and Probe sequences designed by Charité Universitätsmedizin Berlin ; https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf. SOPs in the attached pdf. Disclaimer: This product does not contain the polymerase For RUO (research use only)

Lyophilized encapsidated RNA for SARS-CoV-2 (2019-nCoV) real time RT-PCR targeting region of the E gene, for 100 Rxns (Target designed by Charité Universitätsmedizin Berlin ; https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf). The encapsidated RNA has been stabilized to serve as positive control for extraction and real time RT-PCR. Extraction is mandatory before use in real time RT-PCR. SOPs in the attached pdf. For RUO (Research Use Only)

Lyophilized encapsidated RNA for 2019-nCoV real time RT-PCR targeting region of the E gene (for 100 Rxns) AND Lyophilized ready-to-use primers and probe (Lyoph-P&P) for real time RT-PCR (96 rxns in the form of 4x24 rnxs). Target, primers and probe designed by CUB Berlin. SOPs in the attached pdf. Disclaimer: This product does not contain the polymerase

Infectious cell culture supernatant containing Human 2019-nCoV strain 2019-nCoV/Italy-INMI1. This strain is preserved under Viral Storage Medium -80C. To confirm its identity the virus has been completely sequenced.

RNA of Human 2019-nCoV strain 2019-nCoV/Italy-INMI1. To confirm its identity the virus has been completely sequenced. The complete sequence was submitted to GenBank (SARS-CoV-2/INMI1-Isolate/2020/Italy: MT066156) and is available on GISAID website (BetaCoV/Italy/INMI1-isl/2020: EPI_ISL_410545) upon registration. Import permit can be needed in your country. If yes, please indicate it in your online enquiry.

anti-GP 3 of Porcine reproductive and respiratory syndrome virus (PRRSV) murine monoclonal antibody, clone Hy P10-c40-c1 (CCLV-RIE 1167)

anti-N of Porcine reproductive and respiratory syndrome virus (PRRSV) murine monoclonal antibody, clone Hy P10-a1-10-c19 (CCLV-RIE 1168)

anti-GP 5 of Porcine reproductive and respiratory syndrome virus (PRRSV) strain I10, Hy P10-b38-c16 (CCLV-RIE 1089)

anti-GP 5 of Porcine reproductive and respiratory syndrome virus (PRRSV) strain I10 murine monoclonal antibody, clone Hy P10-a46-15 (CCLV-RIE 1175)

anti-GP 4 of Porcine reproductive and respiratory syndrome virus (PRRSV) murine monoclonal antibody, clone Hy P11.B2-b17 (CCLV-RIE 0375)

Purified RNA of Coronavirus strain "BetaCoV/Germany/BavPat1/2020 p.1" grown in cell culture. Dislaimer: Import permit maybe be needed in your country. If yes, please indicate so in your online enquiry.

Infectious cellculture supernatant of human 2019-nCoV

In vitro transcribed RNA for Wuhan coronavirus (2019-nCoV) real time (RT-)PCR targeting region of the E gene . Protocol and preliminary evaluation for this assays (Corman/Drosten 2020), as of Jan 13, 2020, available from: https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf

Purified RNA of SARS-Coronavirus strain Frankfurt 1, grown in cell culture. Dislaimer: Import permit maybe be needed in your country for the SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry.

Infectious cell culture supernatant of human 2019-nCoV

Panel consists of (cell-culture) RNA from different coronaviruses (HCoV-Nl63, HCoV-229E, HCoV-OC43, MERS-CoV and SARS-CoV) and is intended for use as control of specificity of molecular assays. This panel contains sufficient RNA for t least 5 experiments. "Disclaimer: Import permit may be needed in your country for SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry". Product for Alphacoronavirus and Betacoronavirus. ------------------ NB: indicated price is only valid for non-profit laboratories. Commercial users will be contacted about pricing.

Live virus SARS-Coronavirus Frankfurt 1. This SARS coronavirus Frankfurt 1[Frankfurt-Frankfurt 1.] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative.

The in vitro transcribed RNA is the control for the first-line screening assay termed E gene assay. The SARS-CoV full virus RNA is required to control the two confirmatory assays. Dislaimer: Import permit maybe be needed in your country for the SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry.

Betacoronavirus 1 / Bovine coronavirus, strain "S379 Riems"; host: cattle; origin: Germany, 1986

Through direct cloning and expression of the variable heavy chains (VHHs) of HCAbs from the bone marrow of MERS-CoV infected dromedaries, MERS-CoV specific VHHs or nanobodies were produced. VHH-83 binds with exceptionally high affinity to the receptor binding domain of the viral spike protein. In vitro, this VHH efficiently blocks virus entry at picomolar concentrations

HCAb-83 is a MERS-CoV specific camel/human chimeric heavy chain only antibody (HCAb) - composed of the camel VHH-83 linked to a human Fc domain lacking the CH1 exon Through direct cloning and expression of the variable heavy chains (VHHs) of HCAbs from the bone marrow of MERS-CoV infected dromedaries, MERS-CoV specific VHHs or nanobodies were produced. VHH-83 binds with exceptionally high affinity to the receptor binding domain of the viral spike protein. In vitro, HCAb-83 efficiently blocks virus entry at picomolar concentrations

In this 2-day course the basic principles of routine virus neutralization and ELISA assays will be addressed by a combination of lectures, demonstration and hands-on training. Special attention will be given to quality control and assay validation. Topics include: cell culture, biosafety procedures, preparation of sera and virus stocks, ELISA and microarray formats, different virus neutralization tests using pseudotyped viruses and wildtype viruses, immunofluorescence staining of cells, calculation of titers, assay validation and quality control.

Anti-Middle East respiratory syndrome-related coronavirus NP protein polyclonal rabbit sera

Middle East respiratory syndrome (MERS) Coronavirus, strain IP/COV/MERS/Hu/France/FRA2

Serum obtained from rabbits challenged with MERS-CoV.