EVAg

Lyophilized encapsidated RNA for SARS-CoV-2 (2019-nCoV) real time RT-PCR targeting region of the N (CUB) gene, for 100 Rxns (Target designed by Charité Universitätsmedizin Berlin). The encapsidated RNA has been stabilized to serve as positive control for extraction and real time RT-PCR. Extraction is mandatory before use in real time RT-PCR. SOPs in the attached pdf. For RUO (Research Use Only)

N-terminal domain of the SARS-CoV-2 nucleprotein (1-180). Pure recombinant protein produced in E. coli and fused with a Avi tag and a Hexa-Hisitidine tag.

C-terminal domain of the SARS-CoV-2 nucleprotein (247-418). Pure recombinant protein produced in E. coli and fused with a Avi tag and a Hexa-Hisitidine tag.

Freeze dried SARS-CoV-2 virus, strain UVE/SARS-CoV2/2020/FR/ISA_D614 generated by ISA method. It contains the original D614 residue on the Spike protein

Infectious cell culture supernatant containing SARS-CoV-2 isolate SARS-CoV-2/Human/ITA/PAVIA10734/2020, clade G, D614G (S). This strain is preserved under Viral Storage Medium -80C and is Mycoplasma free.

Infectious cell culture supernatant containing Human 2019-nCoV strain 2019-nCoV/Italy-INMI1, clade V. This strain is preserved under Viral Storage Medium -80C.

Freeze-dried SARS-CoV-2 virus, strain UVE/SARS-CoV-2/2020/FR/702, France 2020, complete genome sequence. NB: this virus contains the G614 european mutation in the spike sequence

Recombinant expressed and purified receptor-binding domain (RBD) of SARS-CoV-2 for serological assays. The Protein was expressed in Expi293 cells and purified via Strep-Tactin XT and provided in Elution Buffer ( 100mM Tris-HCl; pH 8.0, 150mM NaCl, 1mM EDTA, 50mM Biotin).

Recombinant expressed and purified receptor-binding domain (RBD) of SARS-CoV-2 for serological assays. The Protein was expressed in Expi293 cells and purified via a HisTag and provided in Elution Buffer (50 mM NaH2PO4, 300 mM NaCl, 0,05% Tween, 250 mM Imidazole, pH 8.0).

Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 4x24 rxns) for real time RT-PCR of SARS-CoV-2 (2019-nCoV) as of Jan 30, 2020, available from https://www.mdpi.com/1999-4915/12/2/159/pdf. Primers and Probe sequences designed by Charité Universitätsmedizin Berlin ; https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf. SOPs in the attached pdf. Disclaimer: This product does not contain the polymerase For RUO (research use only)

Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 4x24 rxns) for real time RT-PCR of SARS-CoV-2 (2019-nCoV) in the RdRp (IP4) gene. Primers and Probe sequences designed by Institut Pasteur: https://www.who.int/docs/default-source/coronaviruse/real-time-rt-pcr-assays-for-the-detection-of-sars-cov-2-institut-pasteur-paris.pdf?sfvrsn=3662fcb6_2) SOPs in the attached pdf. Disclaimer: This product does not contain the polymerase For RUO (research use only)

This product represents total RNA extracted from the viral stock of SARS-CoV-2 strain Slovakia/SK-BMC5/2020 which is available as a viable virus under Ref-SKU: 006V-03933.

This product represents cDNA material, transcribed with random hexamers from total RNA extracted from the viral stock of SARS-CoV-2 strain Slovakia/SK-BMC5/2020 which is available as a viable virus under Ref-SKU: 006V-03933.

Alphacoronavirus 1 (Transmissible gastroenteritis Virus, TGEV), strain "70" (RVB-1158), Germany 1980

Infectious cell culture supernatant containing SARS-CoV-2 isolate SARS-CoV-2/human/ITA/INMI4/2020, clade GR, D614G (S). This strain is preserved under Viral Storage Medium -80C and is Mycoplasma free.

RNA of Human 2019-nCoV strain 2019-nCoV/Italy-INMI1. The RNA was obtained from a viral strain isolated in Italy, from a sample collected in January 29, 2020. To confirm its identity the virus has been completely sequenced. The complete sequence was submitted to GenBank (SARS-CoV-2/INMI1-Isolate/2020/Italy: MT066156) and is available on GISAID website (BetaCoV/Italy/INMI1-isl/2020: EPI_ISL_410545) upon registration. Import permit can be needed in your country. If yes, please indicate it in your online enquiry.

Infectious cell culture supernatant containing SARS-Cov-2 (strain human/DEU/HH-1/2020). This strain is preserved under Viral Storage Medium -80C. To confirm its identity the virus has been completely sequenced

Infectious cellculture supernatant of human 2019-nCoV

Infectious cell culture supernatant containing SARS-CoV-2 (strain hCoV-19/Netherlands/NoordBrabant_10003/2020, clade B, D614 (S)). This strain is preserved in viral storage medium -80°C.

Infectious cell culture supernatant containing SARS-CoV-2 (strain hCoV-19/Netherlands/ZuidHolland_10004/2020, D614G (S)). This strain is preserved in viral storage medium -80°C.

Infectious cell culture supernatant containing SARS-Cov-2 (strain Slovenia/SI-4265/20, D614G). This strain is preserved under Viral Storage Medium -80C. To confirm its identity the virus has been completely sequenced.

Infectious cell culture supernatant of human 2019-nCoV, clade G with D614G (S)

This SARS-CoV-2 strain was isolated from a COVID-19 patient from Slovakia in March 2020. Its complete sequence is deposited on GISAID.org under the accession ID EPI_ISL_417880.

Training in Coronavirus reverse genetics to adapt novel techniques (ISA method) to large RNA viruses and compare different technologies under BSL-3 conditions

Lyophilized encapsidated RNA for SARS-CoV-2 real time RT-PCR targeting region of the N(CUB) gene (for 100 Rxns) AND Lyophilized ready-to-use primers and probe (Lyoph-P&P) for real time RT-PCR (96 rxns in the form of 4x24 rnxs). Target, primers and probe designed by Charité (CUB) Berlin. SOPs in the attached pdf. Disclaimer: This product does not contain the polymerase