Product Description
Ref-SKU:
029N-05462 Aeonium ringspot virus, RNA1 and RNA2 full-length cDNAs are cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNAs.
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Cloned Product: Yes
Can it be used to produce GMO:
No
Region encompassed in this Product:
complete genome
Plasmid:
Plasmid selection:
TAG:
Sequencing:
Fully sequenced
Sequence checked:
Yes
Mutations:
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations:
Two G residues hve been inserted upstream the 5' nucleotide of the AeRSV sequence in both RNA1 and RNA2 cDNA clones. This allows efficient in vitro transcription. Linearization of RNA1 is obtained by SmaI digestion. Linearization of RNA2 is obtained by BamHI digestion.
Genbank reference:
Nucleic Acid preparation technique:
Plasmid amplification in Escherichia coli DH5alpha. DNA preparation obtained by alkaline lysis, phenol extraction and PEG precipitation
Storage conditions:
Nuclease-Free Water -20C
Shipping conditions:
IATA Classification:
Virus host type:
Information about the origin of the product
Recombinant product:
This product is a recombinant product
Biological material origin:
Synthetic origin & Natural origin
Multiple reference sequences were used from the public databases for the design of this product
Synthetic part identification: Aeonium ringspot virus
Synthetic part identification:
Aeonium ringspot virus
Genbank reference:
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has been modified from the sequence listing provided as Genbank references
Description of modification(s) made from the reference sequence(s):
CLONED IN PUC18. THE 5' TERMINAL A HAS BEEN REPLACED BY G TO ALLOW EFFICIENT T7 DRIVEN TRANSCRIPTION
Natural part identification: Aeonium ringspot virus
Natural part identification:
Aeonium ringspot virus
Collection date:
Sunday, 6 March, 2011
Country of collection: