Product Description
Ref-SKU:
029N-05402 The 3' terminal 217 nts of Aeonium ringspot virus RNA1 and RNA2 (excluding the poly(A) tail) are cloned in pGEM-T Easy allowing for transcription of a riboprobe to detect both genomic RNAs.
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Cloned Product: Yes
Can it be used to produce GMO:
No
Region encompassed in this Product:
partial genome sequence (3' terminal 217 nt)
Plasmid:
Plasmid selection:
TAG:
Sequencing:
Fully sequenced
Sequence checked:
Yes
Mutations:
No mutation compared to the reference sequence
Nucleic Acid preparation technique:
Plasmid amplification in Escherichia coli DH5alpha. DNA preparation obtained by alkaline lysis, phenol extraction and PEG precipitation
Storage conditions:
Nuclease-Free Water -20C
Shipping conditions:
IATA Classification:
Virus host type:
Note:
The clone containing the 3' 217 nucleotides can be linearized by SpeI to generate a riboprobe for the detection of both RNA1 and RNA2.
Information about the origin of the product
Recombinant product:
This product is a recombinant product
Biological material origin:
Synthetic origin & Natural origin
Natural part identification: Plants of Aeonium spp. virus-infected
Natural part identification:
Plants of Aeonium spp. virus-infected
Genbank reference:
Collection date:
Friday, 6 September, 2013
Country of collection:
Synthetic part identification: plasmid DNA
Synthetic part identification:
plasmid DNA
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has NOT been modified from the sequence listing provided as Genbank references