HIV-1 pNL-BX_prK45I-L76F


Product Description

Ref-SKU:
006N-01988
Plasmid contains the entire genome of HIV-1 (NL4-3) with specific mutations in the protease gene, which can confer drug resistance to specific HIV-1 protease inhibitors. The respective mutations have been reported in viruses from HIV-infected individuals on protease inhibitors with virologic therapy failure.
Product Risk Group: 

Information on the related virus

Subspecies: 
Strain: 
Isolate: 


Unit definition: 
10 ng purified DNA

In stock

675,00 €
(Cost per access for Academics)

catalogue

biobank

Portal

EVAg

purchase

buy

order

price

cost

shipping

shipment

experiment

research

laboratory

viral

virus

Cloned Product: Yes
Can it be used to produce GMO: 
No
Biosafety restrictions: 
Plasmid contains the entire genome of HIV-1 (NL4-3) with specific mutations in the protease gene, which can confer drug resistance to specific protease inhibitors. Might produce infectious virus after transfection into suitable human cells.
Region encompassed in this Product: 
entire HIV-1 genome
Plasmid: 
Plasmid selection: 
TAG: 
Sequencing: 
Fully sequenced
Sequence checked: 
Yes
Mutations: 
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations: 
The virus genome is derived from pNL-NF (pNL4-3 with shortened cellular flank) (doi.org/10.1016/j.jcv.2006.05.001). mutations as indicated in the name; backbone is NL-BX. A unique BamHI site was introduced 5’ of protease (ggagcc2207ggaTcc; A->S in Pol; E->D in Gag) and an XmaI site 3’ of protease (ccagga2589ccCggG). The original BamHI site as position 8465 was removed (GGATCC->GGATCA change silent in Env; L->S in Rev). Other mutations as indicated in the product name.
Nucleic Acid preparation technique: 
ion exchange column (Macherey Nagel)
Storage conditions: 
TE Buffer -20C
Shipping conditions: 
IATA Classification: 
Virus host type: 
Note: 

To show the resistance pattern to antiretroviral drugs caused by the mutations present in this viral clone, use e.g. the „mutations list analysis“ function of the public HIV-Grade site at:

www.hiv-grade.de/grade_new/

Information about the origin of the product

Recombinant product: 
This product is a recombinant product
Biological material origin: 
Synthetic origin & Natural origin