Product Description
Ref-SKU:
006N-01922 Recombinant HIV-1 plasmid (derived from pNL4-3) with the indicated mutations in the protease gene, which have been reported in the context of clinical drug resistance to HIV protease inhibitors.
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Subspecies:
Strain:
Isolate:
Genotype:
Cloned Product: Yes
Special feature:
Can it be used to produce GMO:
No
Biosafety restrictions:
Plasmid DNA itself is not infectious unless transfected into suitable cells. Will produce live HIV-1 after transfection into mammalian cells. Infectivity of resulting virus has been verified! The virus has proven drug resistance against clinical HIV protease inhibitors
Region encompassed in this Product:
entire genome of HIV-1
Plasmid:
Plasmid selection:
TAG:
Sequencing:
Fully sequenced
Sequence checked:
Yes
Mutations:
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations:
The virus genome is derived from pNL-NF (pNL4-3 with shortened cellular flank) (doi.org/10.1016/j.jcv.2006.05.001).
mutations as indicated in the name; backbone is NL-BX.
A unique BamHI site was introduced 5’ of protease (ggagcc2207ggaTcc; A->S in Pol; E->D in Gag) and an XmaI site 3’ of protease (ccagga2589ccCggG).
The original BamHI site as position 8465 was removed (GGATCC->GGATCA change silent in Env; L->S in Rev).
Other mutations as indicated in the product name.
Nucleic Acid preparation technique:
Plasmid-Miniprep (Macherey Nagel)
Storage conditions:
TE Buffer -20C
Shipping conditions:
IATA Classification:
Virus host type:
Note:
To show the resistance pattern to antiretroviral drugs caused by the mutations present in this viral clone, use e.g. the „mutations list analysis“ function of the public HIV-Grade site at:
Information about the origin of the product
Recombinant product:
This product is a recombinant product
Biological material origin:
Synthetic origin & Natural origin
Natural part identification: NL4-3
Natural part identification:
NL4-3
Genbank reference:
Collection date: BEFORE
Wednesday, 1 September, 2010
Country of collection:
Synthetic part identification: point mutations at the indicated positions in the protease gene, 10F, 46I, 71V, 82A, 84V
Synthetic part identification:
point mutations at the indicated positions in the protease gene, 10F, 46I, 71V, 82A, 84V
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has been modified from the sequence listing provided as Genbank references
Description of modification(s) made from the reference sequence(s):
PCR-based mutagenesis; carries the indicated mutation(s) in the target gene as shown in the product name