HIV-1 pNL-BX_prL10I

ANTIVIRAL RESISTANT STRAIN/ REFERENCE STRAIN


Product Description

Ref-SKU:
007N-05788
full length plasmid clone of proviral HIV-1; construct produces fully infectious HIV-1 upon transfection into eukaryotic cells; based on NL-BX
Product Risk Group: 

Information on the related virus

Strain: 


Unit definition: 
10 ng DNA

In stock

675,00 €
(Cost per access for Academics)

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Cloned Product: Yes
Can it be used to produce GMO: 
No
Biosafety restrictions: 
Upon transfection of eukaryotic cells DNA can produce infectious virus causing disease in humans
Region encompassed in this Product: 
entire genome
Plasmid: 
Plasmid selection: 
TAG: 
Sequencing: 
Fully sequenced
Sequence checked: 
Yes
Mutations: 
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations: 
The virus genome is derived from pNL-NF (pNL4-3 with shortened cellular flank) (doi.org/10.1016/j.jcv.2006.05.001). mutations as indicated in the name; backbone is NL-BX. A unique BamHI site was introduced 5’ of protease (ggagcc2207ggaTcc; A->S in Pol; E->D in Gag) and an XmaI site 3’ of protease (ccagga2589ccCggG). The original BamHI site as position 8465 was removed (GGATCC->GGATCA change silent in Env; L->S in Rev).
.gb file of the construct: 
Identification technique: 
restriction digest (Hind III), partial sequencing
Nucleic Acid preparation technique: 
solid phase column extraction
Storage conditions: 
TE Buffer -20C
Shipping conditions: 
IATA Classification: 
Virus host type: 
Note: 

To show a possible resistance pattern to antiretroviral drugs caused by the mutations present in this viral clone, use e.g. the „mutations list analysis“ function of the public HIV-Grade site at:

www.hiv-grade.de/grade_new/

Information about the origin of the product

Recombinant product: 
This product is a recombinant product
Biological material origin: 
Synthetic origin & Natural origin