HIV-1 pNL-BX_prL10I-G48V-L63P-A71V-V82A-L90M
nucleic-acid

HIV-1 pNL-BX_prL10I-G48V-L63P-A71V-V82A-L90M

ANTIVIRAL RESISTANT STRAIN/ REFERENCE STRAIN

EVAg
Produced by
: UNIBAS
Shipping From
: Basel - CH

Product Description

Ref-SKU:
007N-05778
full length plasmid clone of proviral HIV-1; construct produces fully infectious HIV-1 upon transfection into eukaryotic cells; based on NL-BX
Product Risk Group: 
RG2

Information on the related virus

ICTV Taxonomy: 
Riboviria / Pararnavirae / Artverviricota / Revtraviricetes / Ortervirales / Retroviridae / Orthoretrovirinae / Lentivirus / Human immunodeficiency virus 1
Virus name: 
Human immunodeficiency virus type 1
Strain: 
subtype B
Isolate: 
derivative of HIV-1 NL4-3


Unit definition: 
10 ng DNA

In stock

675,00 €
 (Cost per access for Academics)
Free access available

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Cloned Product: Yes
Special feature: 
Antiviral resistant strain
Reference Strain
Can it be used to produce GMO: 
No
Biosafety restrictions: 
Upon transfection of eukaryotic cells DNA can produce infectious virus causing disease in humans
Region encompassed in this Product: 
entire genome
Plasmid: 
pUC 18 (Pvu II)
Plasmid selection: 
Ampicillin
TAG: 
No Tag
Sequencing: 
Fully sequenced
Sequence checked: 
Yes
Mutations: 
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations: 
mutation as indicated in the name; backbone is NL-BX: The virus genome is derived from pNL-NF (pNL4-3 with shortened cellular flank) (doi.org/10.1016/j.jcv.2006.05.001), after introduction of a unique BamHI site 5’ (ggagcc2207ggaTcc; A->S in Pol; E->D in Gag) and an XmaI site 3’ (ccagga2589ccCggG; silent in Env; L->S in Rev) from the protease gene, respectively. The original BamHI site as position 8465 was removed (GGATCC->GGATCA change).
Identification technique: 
restriction digest (Hind III), partial sequencing
Nucleic Acid preparation technique: 
solid phase column extraction
Storage conditions: 
TE Buffer -20C
Related DOI: 
original clone NL4-3: DOI: 10.1128/JVI.59.2.284-291.1986
Shipping conditions: 
Room Temperature
IATA Classification: 
NON DANGEROUS GOODS
Virus host type: 
Human virus

Information about the origin of the product

Recombinant product: 
This product is a recombinant product
Biological material origin: 
Synthetic origin & Natural origin

Natural part identification: 
NL4-3 derivative (based on pNL-BX)

Genbank reference: 
AF324493
Collection date: BEFORE 
Friday, 1 January, 2010
Country of collection: 
United States of America (the)

Synthetic part identification: 
based on NL4-3 sequence

Modifications from the reference sequence(s): 
The sequence(s) of this synthetic product has been modified from the sequence listing provided as Genbank references
Description of modification(s) made from the reference sequence(s): 
carries mutations as indicated in product name