- Tagged as
- - In Vitro Transcript
- - Diagnostic reagent
- - Derived product
- - Nucleic Acid
Produced by
: CUB Shipping From
: Berlin - DE
Product Description
Ref-SKU:
004N-EVA1386 In vitro transcribed RNA for new Betacoronavirus virus (hCoV-EMC) real time PCR targeting region upstream of the E gene (upE). Reference: Corman et. Al., Eurosurveillance, Volume 17, Issue 39, 27 September 2012
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Cloned Product: No
Can it be used to produce GMO:
No
Sequencing:
Fully sequenced
Sequence checked:
Yes
Mutations:
No mutation compared to the reference sequence
Genbank reference:
Identification technique:
PCR
Nucleic Acid preparation technique:
Cloning of target region (details provided in Certificate of Analysis) in a pCR4 TOPO vector, reamplification with vector specific primers followed by T7-driven in vitro transcription using MEGAscript (Asuragen, Lifetech). RNA was subsequently purified by QIAGEN RNeasy kit.
Titer:
100000.00 1 µl
Storage conditions:
Nuclease-Free Water -80C
Shipping conditions:
IATA Classification:
Virus host type:
Information about the origin of the product
Biological material origin:
Synthetic origin
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has NOT been modified from the sequence listing provided as Genbank references