Product Description
Ref-SKU:
016N-05981 Plasmid coding the full-length antigenome of Ebola virus isolate Makona from West African outbreak 2014. The plasmid can be used for the rescue of recombinat virus.
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Subspecies:
Isolate:
Cloned Product: Yes
Can it be used to produce GMO:
Yes
Biosafety restrictions:
The plasmid can be used to rescue recombinant RG4 virus, it will be provided only to operating BSL4 laboratories
Region encompassed in this Product:
full-lenght antigenome of Ebola virus strain Makona
Plasmid:
Plasmid selection:
TAG:
Sequencing:
Fully sequenced
Sequence checked:
Yes
Mutations:
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations:
introduced genetic marker (FspI restriction site) Pos 14149 C>T silent in the L-gene
Genbank reference:
Identification technique:
sequencing and enzymatic restriction
Nucleic Acid preparation technique:
Qiagen Maxi-prep kit
Storage conditions:
TE Buffer -20C
Shipping conditions:
IATA Classification:
Virus host type:
Information about the origin of the product
Recombinant product:
This product is a recombinant product
Biological material origin:
Synthetic origin & Natural origin
Synthetic part identification: plasmid minimal vector with an ampicillin resistence gene
Synthetic part identification:
plasmid minimal vector with an ampicillin resistence gene
Genbank reference:
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has NOT been modified from the sequence listing provided as Genbank references
Natural part identification: sequence of the Ebola virus isolat Makona
Natural part identification:
sequence of the Ebola virus isolat Makona
Genbank reference:
Collection date: BEFORE
Thursday, 18 December, 2014
Country of collection: