EVAg

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect castor bean tick nymphs (Ixodes ricinus). The nymphs will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 nymphs will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 nymphs and provided to the end-user.

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect adult castor bean ticks (Ixodes ricinus). The ticks will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 ticks will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 ticks and provided to the end-user.

Access to RIVM infrastructure to use anonymized human fecal material containing fully sequenced norovirus strains, representing different genotypes. Strains/genotypes/year (2012-2023) selection on demand. Custom-made package includes individual fecal material from 5 different patients, 10-20% suspended in virus culture medium. Service includes technical input on protocols and experiment design and support in the laboratory. Individual questions to refine potential requests can be sent to RIVM-EVAglobal@rivm.nl.

Access to RIVM infrastructure to use anonymized human fecal material containing fully sequenced sapovirus strains, representing different genotypes. Strains/genotypes/year (2012-2023) selection on demand. Custom-made package includes individual fecal material from 2 different patients, 10-20% suspended in virus culture medium. Service includes technical input on protocols and experiment design and support in the laboratory. Individual questions to refine potential requests can be sent to RIVM-EVAglobal@rivm.nl.