EVA

HEK293T-indE (HEK293T-E Tet:E-IRES-ORF6) cells are a derivative of HEK293T-E with a second-generation lentiviral vector generated with the pCW57-E-IRES-ORF6 (Addgene plasmid #80921) as a transfer vector. The vector codes for SARS-COV-2 E and ORF6 under a Tetracycline inducible promoter. Cell line is needed for recovery of ∆E-based vaccine virus, which needs to be propagated on E-transcomplementing cells.

Vero-E2T cells express SARS-CoV-2 E/ORF6/ORF7a/ORF8 genes, under the CMV promoter. Human TMPRSS2 expression in Vero-E2T was achieved by infecting the cells with a 2nd generation lentiviral vector pLEX307-TMPRSS2-blast (Addgene plasmid #158458) as a transfer vector. This cell line is needed for SARS-CoV-2 ∆E vaccine virus propagation.

The Public Health Laboratories of Hellenic Pasteur Institute (HPI) invite laboratory professionals to an intensive 4-day hands-on training course offering cutting-edge Next-Generation Sequencing (NGS) training for Respiratory Syncytial Virus (RSV), using Oxford Nanopore Technology. This program combines high-quality sequencing analysis with practical training modules, enabling participants to confidently implement and interpret RSV genomic analysis in their home laboratories.

The Public Health Laboratories of Hellenic Pasteur Institute (HPI) invite laboratory professionals to an intensive 4-day hands-on training course offering cutting-edge Next-Generation Sequencing (NGS) training for Influenza Virus, using Oxford Nanopore Technology. This program integrates high-quality sequencing analysis with hands-on training, providing skills to generate, analyze, and interpret Influenza genomic data for research, surveillance, and diagnostics.

The Public Health Laboratories of Hellenic Pasteur Institute (HPI) offer a comprehensive 5-day training course on Next-Generation Sequencing (NGS) for Influenza and Respiratory Syncytial Virus (RSV), using Oxford Nanopore Technology. This course is designed to provide participants with the knowledge and practical skills required to perform whole-genome sequencing and analysis of two major respiratory viruses—Influenza and RSV—supporting surveillance, research, and diagnostic development.

The Public Health Laboratories of Hellenic Pasteur Institute (HPI) invite laboratory professionals to participate in an intensive 3-day hands-on training course designed to enhance laboratory expertise in molecular diagnostics of viral infections. This comprehensive program covers the full workflow of assay validation and implementation, combining theory with practical insights. The training will be tailored to the trainee’s needs and may include: 1. Detection and typing of key viruses, 2.

Molecular panel of different arboviruses (flaviviruses, alphaviruses and orthobunyaviruses) for EQA, containing 16 freeze-dried vials.

Virus whole genome sequencing

Course instruction will be conducted in the high containment facility at ACDP, Geelong, Victoria, Australia. Participants will receive a 5-day intensive course that includes interactive theoretical and practical experiences. Topics addressed will include introduction to BSL4 suited laboratories, regulatory frameworks, operational oversight, engineering controls and maintenance considerations.

An in-depth theoretical and practical application of biosafety, biocontainment and biosecurity under the Australia/New Zealand standards and Australian regulatory framework for Biosecurity Containment and Physical Containment level 3 and 4 laboratory and animal facilities.

During visit of the facility examples for comprehensive detection and measures to control quarantine pathogens (viruses, bacteria, fungi, nematodes) will be presented and the associated quality management will be demonstrated. Furthermore time for mutual scientific exchange and discussions on the control of known and emerging viruses is given. The number of applicants is limited to one to two persons per visiting period.

High-throughput sequencing has developed into a versatile standard tool in plant virology research. It provides fast and precise genomic data on the viruses being processed for a wide range of applied and basic research questions. This service package offers the individual analysis of three plant samples by HTS to ensure a reliable detection and assembly of the viral genomes.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect castor bean tick nymphs (Ixodes ricinus). The nymphs will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 nymphs will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 nymphs and provided to the end-user.

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect adult castor bean ticks (Ixodes ricinus). The ticks will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 ticks will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 ticks and provided to the end-user.

Virus production of viruses available on the EVAg catalogue from BMC-SAS in a single batch upon request containing 30 cryotubes containing 0.5mL.

Virus production of viruses available on the EVAg catalogue from BMC-SAS in a single batch upon request containing 30 cryotubes containing 0.5mL.

Human Immunodeficiency Virus (HIV) drug resistance remains a significant health issue that challenges treatment and prevention. The objective of this 3-week course is to learn bioinformatics techniques for the analysis of next-generation HIV sequencing results. This covers both HIV-1 and HIV-2. Teaching objectives include data curation, alignment, phylogenetics for typing, identification of drug resistance mutations, production of a resistance score, and interpretation. This course does not include genotyping itself, only sequence analysis and interpretation, as described above.

We offer a 5 day training session to colleagues from endemic regions. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for the quantification rabies virus and neutralizing antibodies.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

We would like to invite African researchers to a training workshop on virus diagnostics. In the scope of this three-day workshop, participants from various African countries will acquire hands-on experience in the detection and characterisation of viruses using electron microscopy and Reverse Transcription Polymerase Chain Reaction (RT-PCR). This workshop offers a valuable opportunity for participants to connect and to network with each other, contributing to the prevention of virus outbreaks.

The detection of plant viruses in environmental samples, such as insects, mites, crops, weeds. obtained through high-throughput sequencing (HTS) followed by appropriate bioinformatics analyses, allows to verify the phytosanitary status of plants and is particularly useful for the identification of viruses involved in diseases with unknown etiology. The service offers the individual analysis of three samples by HTS and the downstream bioinformatic analysis, up to the identification of sequences of viral origin in each sample.

This four day course will go through the general principles for the diagnosis of bovine leukemia virus, through a combination of discussions, demonstrations and hands-on training. The training will include sample processing, PCR, ELISA (serology), reagent production, virus related bio-safety and diagnostic quality systems.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.