EVAg

The detection of plant viruses in environmental samples, such as insects, mites, crops, weeds. obtained through high-throughput sequencing (HTS) followed by appropriate bioinformatics analyses, allows to verify the phytosanitary status of plants and is particularly useful for the identification of viruses involved in diseases with unknown etiology. The service offers the individual analysis of three samples by HTS and the downstream bioinformatic analysis, up to the identification of sequences of viral origin in each sample.

The detection of viral pathogens infecting a given plant sample, obtained through high-throughput sequencing (HTS) followed by appropriate bioinformatics analyses, allows to verify the phytosanitary status of plants and is particularly useful for the identification of viruses involved in diseases with unknown etiology or for the certification of seed and planting material. The service offers the individual analysis of three plant samples by HTS and the downstream bioinformatic analysis, up to the identification of sequences of viral origin in each sample.

In this two-week course, to be held in the CNR-IPSP laboratories in Torino (Italy), the basic principles of geminivirus typing and molecular characterization will be addressed by a combination of lectures, demonstration and hands-on training. Topics include: sample preparation, nucleic acid extraction, rolling-circle amplification, RFLP analysis, cloning, sequencing, phylogenetic analysis.

In this five-day course, to be held in the CNR-IPSP laboratories in Torino (Italy), the basic principles of geminivirus diagnosis will be addressed by a combination of lectures, demonstration and hands-on training. Topics include: tissue-printing hybridization, dot-blot hybridization, preparation of non-radioactive probes, rapid sample extraction. After this course participants should have sufficient knowledge to introduce routine diagnostics of geminiviruses in their own laboratory.