EVAg

in vitro transcribed RNA for a specific Yellow fever virus real time PCR

Yellow fever virus plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Purified Recombinant Envelope Domain III Protein (N-terminal SNAP-Tagged, C-terminal His-Tagged) expressed in Drosophila S2 Cells

Viral RNA extracted from infected cell culture lysate.

In vitro transcribed RNA for a specific yellow fever real time RT-PCR (Domingo C. et al, JCM, 2012). Product only available via TNA.

Quantified freeze-dried genomic standard for molecular detection of yellow fever virus, prepared from an inactivated cell culture supernatant of strain YFV-17D with quantified genomic titre. Product only available via TNA.

Recombinant protein produced in E. coli and purified to homogeneity. Fused with a Hexa-Hisitidine tag.

Infectious Subgenomic Amplicons Reverse genetic system for chimeric virus CH-17D / ZIKV. It consists of three non infectious overlapping DNA fragments (cloned into pUC57 vector) . These products enable to obtain infectious RNA virus after amplification by PCR, and transfection into susceptible cells. The chimeric virus systems were constructed using the yellow fever 17-D vaccine strain as a genetic backbone and by replacing prM/E from this vaccine strain with those of the Asian ZIKV PF epidemic strain.

Viral RNA extracted from infected cell culture lysate