Product Description
Ref-SKU:
026V-03883 Infectious cellculture supernatant of human 2019-nCoV
Product Risk Group:
ICTV Taxonomy:
Virus name:
Strain:
Isolate:
Storage conditions:
Viral Storage Medium -80C
Sequencing:
Complete genome
Infectivity:
Infectivity tested and quantified
Mycoplasmic content:
Mycoplasma free
GMO:
No
Virus host type:
Previous Name or Taxonomy:
Infectivity Test:
Infection with MOI of 0,001 leads to a peak in viral load within 48 to 72 hours
Production cell line:
Virus Cultivability:
Passage:
1
Technical recommendation:
Growth Pro: Adherent/ culture at 37°C and 5% CO2
Shipping conditions:
IATA Classification:
Note:
Culture Medium: DMEM (Dulbecco's Modified Eagles Medium) (PAA, Cölbe, Germany) with 4.5 g/L Glucose (PAA) supplemented with 10% Foetal Bovine Serum (PAA), 1% Penicillin/Streptomycin 100 x concentrate (Penicillin 10000 U/mL, Streptomycin 10 mg/ mL) (PAA), 1% L-Glutamine 200 mM, 1% Sodium Pyruvate 100 mM (PAA), 1% MEM nonessential amino acids (NEAA) 100 x concentrate (PAA) - Subculture routine: Detachment with an EDTA/trypsin mixture (PAA) - Split Ratio: Split 1:10 twice a week