Myxoma leporipoxvirus, strain BS/248/92, Italy


EVAg

Originator
:
https://www.izsler.it/
Produced by
: IZSLER
Shipping From
: Brescia - IT

Product Description

Ref-SKU:
026V-04382
The virus has been isolated from rabbit and propagated on RK13 cell line
Product Risk Group: 

Storage conditions: 
Viral Storage Medium -80C
Sequencing: 
Complete genome
Infectivity: 
Infectivity tested and quantified
Mycoplasmic content: 
Mycoplasma free

Unit definition: 
1 mL

In stock

550,00 €
(Cost per access for Academics)

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GMO: 
No
Biosafety restrictions: 
Human Risk Group 1, Animal Risk Group 2
Virus host type: 
Previous Name or Taxonomy: 
Infectivity Test: 
TCID_50mL
Viral titer: 
2x10^7
Production cell line: 
Genbank reference: 
Virus Cultivability: 
Passage: 
10 x RK13
Identification technique: 
PCR, sequencing
Technical recommendation: 
PCR, sequencing
Shipping conditions: 
IATA Classification: 

Information about the collection of the virus

Recombinant product: 
No
Biological material origin: 
Natural origin
Collection date: BEFORE 
Tuesday, 7 April, 1992
Country of collection: 
Suspected epidemiological origin : 
Isolation host: 
Isolation technique: 
Propagation on RK13 cell line
Isolation conditions: 
RK13, cells were maintained in minimal essential medium (MEM) supplemented with 10% calf serum, 2 mM L-glutamine, 500 g/mL amphotericinB, 60g/mL penicillin, 100g/mL streptomycin. For viral isolation 24-well plate of cells was incubated for two hours with 200ml of skin homogenate (10% w/v, PBS1X). At the end the cells were washed twice with PBS1X containing antibiotics and 1ml of complete medium was added to each well.
Clinical information: 
Presence of nodules in the skin