This was originally supposed to be isolated in 1975 from a frog Rana ridibunda (Kozuch et al., Acta virol. 22: 78, 1978), isolated and passaged on suckling mice and stored freeze-dried. This Sindbis virus[183-183-R2] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Product Risk Group:
Viral Storage Medium -80C
Infectivity tested and quantified
Infectivity was proven by plaque titration. Determined virus titer was 1,5x 10^6 PFU/ml.
Production cell line:
Passaged 7 times on suckling mice in 1980 and stored freeze-dried. New stock prepared by one passage on Vero E6 cells on 8th december 2010.
RT-PCR followed by sequencing of the amplified product
We recommend to grow the virus on VeroE6 cells cultivated in MEM Eagle with Earle"s BSS (BioWhittaker, 12-136F). Following additives should be added to 500 ml of medium: 5ml Nonesential amino acids (Biochrom AG, K0293), 5 ml Sodium Pyruvate 100mM, (Biochrom AG, L0473), 5 ml L-Alanyl-L-glutamine 200mM (Biochrom AG, K0302), 5 ml Penicilin/ Streptomycin/ Amphotericin (BioWhittaker 17-745E), 25 ml FBS heat inact.s.American (Invitrogen, 10108-165). Multiplicity of infection (MOI) of 0,001 can be used for the infection and the infectious medium can be harvested 72hours after infection.
Information about the collection of the virus
Biological material origin:
Tuesday, 1 January, 1980
Country of collection: