proviral HIV-1 clone with an inserted TAATGA double stop codon in the endodomain of gp41 after position 8368 (NL4-3); (a stop codon at analogous position have been reported in early reports of SIV propagation in vitro.)
Deletion mutant of HIV-1 with a deleted env gene; RRE reintroduced for the expression of HIV-1 Gag. The genes vpr, env, tat, rev, vpu, nef are not expressed.
Mutant of HIV-1 with deletions between SphI and SalI of pNL4-3, resulting in a frameshift in the gag gene; TAG stop codon inserted in gp41, truncating the endodomain
Double-stranded DNA copy of a coxsackievirus A17 genome without the capsid-encoding region, cloned into a plasmid backbone downstream a T7 promoter. After in vitro transcription, the corresponding RNA can be transfected into cells to give rise to a replicon that is self-replicative and can express the non-structural virus proteins but is unable to produce infectious particles. The plasmid can be amplified in E. coli.
C-terminal domain of the MERS CoV nucleprotein (239-413). Pure recombinant protein produced in E. coli and fused with a Avi tag and a Hexa-Hisitidine tag.
C-terminal domain of the HCoV NL63 nucleprotein (221-367). Pure recombinant protein produced in E. coli and fused with a Avi tag and a Hexa-Hisitidine tag.
