EVA

Template for recombinant SARS-CoV-2 rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), C-terminal tagging of orf8 with 3xFLAG, separated by a flexible linker (GGGGS). Use as fragment D2, S gene not sequence verified. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Template for recombinant SARS-CoV-2 rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), C-terminal tagging of orf7b with 3xFLAG, separated by a flexible linker (GGGGS). Use as fragment D2, S gene not sequence verified. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Template for recombinant SARS-CoV-2 rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), N-terminal tagging of orf7a with 3xFLAG, separated by a flexible linker (GGGGS). Use as fragment D2, S gene not sequence verified. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Template for recombinant SARS-CoV-2 rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), C-terminal tagging of orf6 with 3xFLAG, separated by a flexible linker (GGGGS). Use as fragment D2, S gene not sequence verified. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Template for recombinant SARS-CoV-2 rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), C-terminal tagging of orf3a with 3xFLAG, separated by a flexible linker (GGGGS). Use as fragment D2, S gene not sequence verified. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Template for recombinant SARS-CoV-2 rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, orf6, orf7a and orf8. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035). Only Fragment D2 sequence verified.

Template for recombinant SARS-CoV-2 rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), encodes Wuhan Spike. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

HIV-1 5'LTR Plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Human herpesvirus 6 plasmid for real time PCR

HCV X-tail Plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

HCV 5`ncr Plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Human Coronavirus OC43 plasmid for in vitro transcription

Hepatitis A virus plasmid for in vitro transcription

Hanta virus plasmid for in vitro transcription. The target region of a specific PCR assay was amplified and cloned.

Human Adenovirus plasmid for real time PCR. The target region of a specific real time assay was amplified and cloned.

Influenza A virus plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Enterovirus plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Epstein-Barr-Virus plasmid for real time PCR. The target region of a specific real time assay was amplified and cloned.

Target region of a specific real time assay was amplified and cloned.

Dengue virus serotype 3 plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Dengue virus serotype 4 plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Dengue virus serotype 1 plasmid for invitro transcription - Target region of a specific real time assay was amplified and cloned.

Target region of a specific real time assay was amplified and cloned.

Herpes simplex virus Plasmid for real time PCR. The target region of a specific real time assay was amplified and cloned.

Plasmid for JC virus real time PCR. The target region of a specific real time assay was amplified and cloned.