EVAg

Zika virus, strain 800/16. This Zika virus is freeze dried. Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been fully sequenced.

cDNA of Zika virus isolate Brazil/2016/INMI1, obtained from reverse transcription with non specific hexamers primers

Freeze-dried human plasma or urine spiked with heat inactivated Zika virus and co-circulating arboviruses culture supernatant, (non infectious)

Infectious cell culture supernatant containing Zika virus isolate Brazil/2016/INMI1. This Zika virus strain is preserved under Viral Storage Medium -80C and is Mycoplasma free.

Set of primers and probes to detect viral RNA of Zika virus on diagnostic level. Suitable with the EVD-labnet / EVAg EQA of molecular diagnostics on Chikungunya, Dengue, West Nile, Yellow fever and Zika virus 2019.

Purified Recombinant Envelope Domain III Protein (N-terminal SNAP-Tagged, C-terminal His-Tagged) expressed in Drosophila S2 Cells

Zika virus, strain 800/16. This Zika virus is preserved under Viral Storage Medium -80C. Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been fully sequenced.

Viral RNA from laboratory Zika strain MP1751 infected veros,

Zika virus, strain 976 Uganda. This Zika virus[[976 Uganda]] is freeze dried. Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been fully sequenced.

Freeze-dried Zika virus, strain UVE/ZIKV/2013/PF/Papeete 7, French Polynesia 2013, complete genome sequence

Real time detection kit for Zika virus targeting the envelope gene. The kit contains positive control, primers and probe for 50 reactions. Real time primers and probe were published by “Lanciotti RS, Kosoy OL, Laven JJ, Velez JO, Lambert AJ, Johnson AJ, et al. Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007. Emerg Infect Dis. 2008 Aug;14(8):1232-9.”

ZIka virus, laboratory strain.

Zika virus, strain 976 Uganda. This Zika virus[[976 Uganda]] is preserved under Viral Storage Medium -80C. Tests for the presence of mycoplasmae were negative.

Subgenomic Amplicons for Zika virus strain Dak84 (derived from genbank sequence KU955592). It consists of three non infectious overlapping DNA fragments (amplicons). These products enable to obtain infectious RNA virus after transfection into cells

A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced an in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 638-727 Insert Sequence: 37-637

Infectious Subgenomic Amplicons Reverse Genetic system for Zika virus strain Dak84 (derived from genbank sequence KU955592). It consists of three non infectious overlapping DNA fragments (cloned into pUC57 vector). These products enable to obtain infectious RNA virus after amplification by PCR, and transfection into cells,

A specific genome region was amplified by PCR, cloned into a vector and in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 632-721 Insert Sequence: 37-631

Infectious Subgenomic Amplicons Reverse genetic system for Zika virus strain H/PF/2013. It consists of three non infectious overlapping DNA fragments (cloned into pUC57 vector). These products enable to obtain infectious RNA virus after amplification by PCR, and transfection into cells,

A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced and in vitro transcribed. In vitro transcript: Vector Sequence: Position 1-36, 727-816 Insert Sequence: Position 37-726

Quantified freeze-dried genomic standard for molecular detection of Zika virus, made of an inactivated culture supernatant of strain H/PF/2013 (full sequence of the virus strain KJ776791.1) with quantified genomic titre.

RNA isolation from Viral Stock

Quantified freeze-dried genomic standard for molecular detection of Zika virus, made of an inactivated culture supernatant of strain MR766 with quantified genomic titre

cDNA of Zika virus, strain H/PF/2013, obtained from reverse transcription with non specific hexamers primers

Expression plasmid for the production of the Nterminal - Domain of ZIKV NS5 (methyltransferase domain). Synthetic gene optimized for E. coli. Coding sequence fused at its 5' with a 6His tag coding sequence

RNA extracted from infected cell culture supernatant