HIV-1 pNL-BX_pr-InsAAT(Asn)-at25

ANTIVIRAL RESISTANT STRAIN/ REFERENCE STRAIN


Product Description

Ref-SKU:
007N-05781
full length plasmid clone of proviral HIV-1; construct produces HIV-1 proteins upon transfection into eukaryotic cells but is non-infectious due to a protease-inactivating mutation in the catalytic centre of the protein (D25N); virus is based on NL-BX
Product Risk Group: 

Information on the related virus

Strain: 


Unit definition: 
10 ng DNA

In stock

675,00 €
(Cost per access for Academics)

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Cloned Product: Yes
Can it be used to produce GMO: 
No
Biosafety restrictions: 
Upon transfection of eukaryotic cells DNA can produce infectious virus causing disease in humans
Region encompassed in this Product: 
entire genome
Plasmid: 
Plasmid selection: 
TAG: 
Sequencing: 
Fully sequenced
Sequence checked: 
Yes
Mutations: 
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations: 
mutation as indicated in the name; backbone is NL-BX: The virus genome is derived from pNL-NF (pNL4-3 with shortened cellular flank) (doi.org/10.1016/j.jcv.2006.05.001), after introduction of a unique BamHI site 5’ (ggagcc2207ggaTcc; A->S in Pol; E->D in Gag) and an XmaI site 3’ (ccagga2589ccCggG; silent in Env; L->S in Rev) from the protease gene, respectively. The original BamHI site as position 8465 was removed (GGATCC->GGATCA change). The inserted Asn codon at position 25 of protease renders the virus protease-defective
.gb file of the construct: 
Identification technique: 
restriction digest (Hind III), partial sequencing
Nucleic Acid preparation technique: 
solid phase column extraction
Storage conditions: 
TE Buffer -20C
Shipping conditions: 
IATA Classification: 
Virus host type: 

Information about the origin of the product

Recombinant product: 
This product is a recombinant product
Biological material origin: 
Synthetic origin & Natural origin