EVA

In vitro-transcript of the full coding sequence of the BDBV NP gene, Length 2.223 kb and In vitro-transcript of the full coding sequence of the BDBV L gene, Length 6.637 kb

In vitro-transcript of a short part of the sequence of the BDBV L gene, Length 0.306 kb, Position 13,654-13,957. Suitable for usage in the BDBV EURL-PHAS L and N assay

In vitro-transcript of a short part of the sequence of the BDBV NP gene, Length 0.322 kb, Position 847-1165. Suitable for usage in the BDBV EURL-PHAS L and N assay

RNA extracted from infected cell culture supernatant of Zika virus strain MRS_OPY_Martinique_PaRi_2015. Virus Provenance Martinique 2015,

genomic RNA from culture supernatant of West Nile virus strain UVE/WNV/1999/US/NY 385-99 50µL provided in AVE buffer (without RNA carrier), contains 2.15 x 10^8 RNA cp/µl

genomic RNA from culture supernatant of West Nile virus strain UVE/WNV/1940/UG/UG 956 D117 50µL provided in AVE buffer (without RNA carrier), contains 1.01 x 10^8 RNA cp/µl

Genomic RNA from culture supernatant of Usutu virus strain UVE/USUV/2023/FR/CNR_Cx36-2 50µL provided in AVE buffer (without RNA carrier).

genomic RNA from culture supernatant of Mayaro virus strain UVE/MAYV/1954/TT/TC625. 50µL provided in AVE buffer (without RNA carrier).

RNA extracted from infected cell culture supernatant. Virus Provenance: St Martin, French Caribbean

Viral RNA extracted from infected cell culture lysate

Puumala virus RNA extracted from naturally infected Myodes glareolus - low viral load

RNA extracted from cell culture supernatant

Puumala virus RNA extracted from naturally infected Myodes glareolus - high viral load

RNA extracted from infected cell culture supernatant

Template for chimeric SARS-CoV-2 rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of E, orf6, orf7a and a premature stop codon at position 213 in orf3a. The VSV-G gene is introduced at the locus of orf7a. ∆E-based viruses need to be rescued and propagated on E-transcomplementing cells. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Template for chimeric SARS-CoV-2 rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of E, orf6, orf7a, orf3a. The VSV-G gene is introduced instead of orf3a/E. ∆E-based viruses need to be rescued and propagated on E-transcomplementing cells. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Template for chimeric SARS-CoV-2 rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of E, orf6, orf7a and a premature stop codon at position 213 in orf3a. The VSV-G gene is introduced upstream of N, separated by P2A. ∆E-based viruses need to be rescued and propagated on E-transcomplementing cells. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Template for chimeric SARS-CoV-2 rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of S, E, orf6, orf7a and a premature stop codon at position 213 in orf3a. The VSV-G gene is introduced at the locus of Spike. ∆E-based viruses need to be rescued and propagated on E-transcomplementing cells. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Template for recombinant SARS-CoV-2 rescue; encodes fragment D1 of SARS-CoV-2 (nt20950-25522), Wuhan lineage. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Expression plasmid, encodes SARS-CoV-2 orf8 under the CMV promoter, C-terminal tagged with 3xFLAG, separated by a flexible linker (GGGGS). Designed as control plasmid for endogenously FLAG-tagged SARS-CoV-2 (available as plasmid 007N-06344 or recombinant virus 007V-06299).

Expression plasmid, encodes SARS-CoV-2 orf7b under the CMV promoter, C-terminal tagged with 3xFLAG, separated by a flexible linker (GGGGS). Designed as control plasmid for endogenously FLAG-tagged SARS-CoV-2 (available as plasmid 007N-06343 or recombinant virus 007V-06298).

Expression plasmid, encodes SARS-CoV-2 orf7a under the CMV promoter, N-terminal tagged with 3xFLAG, separated by a flexible linker (GGGGS). Designed as control plasmid for endogenously FLAG-tagged SARS-CoV-2 (available as plasmid 007N-06342 or recombinant virus 007V-06297).

Expression plasmid, encodes SARS-CoV-2 orf6 under the CMV promoter, C-terminal tagged with 3xFLAG, separated by a flexible linker (GGGGS). Designed as control plasmid for endogenously FLAG-tagged SARS-CoV-2 (available as plasmid 007N-06341 or recombinant virus 007V-06296).

Expression plasmid, encodes SARS-CoV-2 orf3a under the CMV promoter, C-terminal tagged with 3xFLAG, separated by a flexible linker (GGGGS). Designed as control plasmid for endogenously FLAG-tagged SARS-CoV-2 (available as plasmid 007N-06340 or recombinant virus 007V-06295).

Template for recombinant SARS-CoV-2 rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), C-terminal tagging of orf6 with BirA, separated by a flexible linker (GGGGS). Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035). Functionality of BirA in rescued virus not tested.