Originator
: Adachi et al, J Virol. 1986 Aug;59(2):284–291
Produced by
: UNIBAS Shipping From
: Basel - CH
Product Description
Ref-SKU:
006N-01920 recombinant HIV-1 virus derived from pNL4-3 carrying (instead of the HIV-1 protease) the protease gene of HIV-2 (ROD14)
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Subspecies:
Isolate:
Genotype:
Cloned Product: Yes
Can it be used to produce GMO:
Yes
Biosafety restrictions:
Plasmid DNA itself is not infectious unless transfected into suitable cells; current studies suggest that the construct leads to an abortive infection
Region encompassed in this Product:
entire virus genome
Plasmid:
Plasmid selection:
TAG:
Sequencing:
Fully sequenced
Sequence checked:
Yes
Mutations:
No mutation compared to the reference sequence
Nucleic Acid preparation technique:
solid phase ion exchange column purification
Storage conditions:
TE Buffer -20C
Shipping conditions:
IATA Classification:
Virus host type:
Note:
The recombinant plasmid has been generated by replacing in frame the original HIV-1 protease gene of NL4-3 with the entire protease gene taken from HIV-2 ROD. The resulting plasmid expresses, upon transfection of human cells, all viral proteins but failed to produce infectious particles in experimental cell infections in vitro.
Information about the origin of the product
Recombinant product:
This product is a recombinant product
Biological material origin:
Synthetic origin & Natural origin
Natural part identification: NL4-3
Natural part identification:
NL4-3
Genbank reference:
Collection date: BEFORE
Wednesday, 1 September, 2010
Country of collection:
Synthetic part identification: pNL4-3 carrying (instead of the HIV-1 protease) the protease gene of HIV-2 (ROD14)
Synthetic part identification:
pNL4-3 carrying (instead of the HIV-1 protease) the protease gene of HIV-2 (ROD14)
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has been modified from the sequence listing provided as Genbank references