JEV


EVAg

Produced by
: BMC-SAS
Shipping From
: Bratislava - SK

Product Description

Ref-SKU:
008v-EVA357
Japanese encephalitis virus - original isolate. This Japanese encephalitis virus[Japanese encephalitis] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Product Risk Group: 
Strain: 
Japanese encephalitis

Storage conditions: 
Viral Storage Medium -80C
Sequencing: 
Partial sequence
Infectivity: 
Infectivity tested and quantified
Mycoplasmic content: 
Mycoplasma free

Unit definition: 
1 ml (2 vials of 0,5ml)

In stock

2 000,00 €
(Cost per access for Academics)

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GMO: 
No
Infectivity Test: 
Infectivity was proven by plaque titration. Infectious titre was determined to be 1x 10^6 PFU/ml.
Viral titer: 
1000000 PFU/ml
Production cell line: 
Shipping conditions: 
Passage: 
Passaged on suckling mice in 1987 and stored freeze-dried. New stock prepared by one passage on Vero E6 cells on 30th May 2011.
IATA Classification: 
Identification technique: 
RT-PCR followed by sequencing of the amplified product.
Technical recommendation: 
We recommend to grow the virus on VeroE6 cells cultivated in MEM Eagle with Earle"s BSS (BioWhittaker, 12-136F). Following additives should be added to 500 ml of medium: 5ml Nonesential amino acids (Biochrom AG, K0293), 5 ml Sodium Pyruvate 100mM, (Biochrom AG, L0473), 5 ml L-Alanyl-L-glutamine 200mM (Biochrom AG, K0302), 5 ml Penicilin/ Streptomycin/ Amphotericin (BioWhittaker 17-745E), 25 ml FBS heat inact.s.American (Invitrogen, 10108-165). Multiplicity of infection (MOI) of 0,001 can be used for the infection and the infectious medium can be harvested 96 hours after infection.

Information about the collection of the virus

Collection date: 
Monday, 30 May, 2011