- Tagged as
- - Antigen
- - Diagnostic reagent
- - Virus
- - Cultivable virus
Produced by
: ERASMUS-MC Shipping From
: Rotterdam - NL
Product Description
Ref-SKU:
010V-05668 Infectious cell culture supernatant containing SARS-CoV-2, JN.1.19. Isolated from throat/Nasopharynx and cultured on Calu-3 cells. To confirm the identity the virus has been sequenced as well as confirmed to be mycoplasma free.
Product Risk Group:
ICTV Taxonomy:
Virus name:
Genotype:
Storage conditions:
Viral Storage Medium -80C
Sequencing:
Complete genome
Infectivity:
Infectivity tested
Mycoplasmic content:
Mycoplasma free
GMO:
No
Biosafety restrictions:
Biosafety lab class 3 requierd
Virus host type:
Infectivity Test:
Tissue Culture Infectious Dose (TCID50) determined using titration assay with Spearman-Karber method. Viral titer: 3,34E+10 TCID50/ml
Production cell line:
Virus Cultivability:
Passage:
3
Identification technique:
PCR, sequencing technology (MinION).
Technical recommendation:
Culture conditions: 37°C and 5% CO2. Culture Medium: Advanced DMEM (Dulbecco's Modified Eagle Medium) supplemented with 1% HEPES (1M), 1% GlutaMax (200 mM), 1% Penicilline/Streptomycine (10000 U/ml ; 10000 µg/ml) and 1% Amphotericine B (0.25 mg/ml).
Shipping conditions:
IATA Classification:
Information about the collection of the virus
Recombinant product:
No
Biological material origin:
Natural origin
Collection date:
Tuesday, 28 November, 2023
Country of collection:
Isolation host:
Isolation technique:
cell culture