SARS-CoV-2, strain hCoV-19/Netherlands/NH-RIVM-27142/2021, Delta variant, lineage B.1.617.2, INACTIVATED


EVAg

Produced by
: RIVM
Shipping From
: Bilthoven - NL

Product Description

Ref-SKU:
014V-04483
Inactivated cell culture supernatant containing SARS-CoV-2 (hCoV-19/Netherlands/NH-RIVM-27142/2021, Delta variant). Virus was heat-inactivated for 2 hours at 60C. This strain is preserved in viral storage medium -80°C.
Product Risk Group: 
Virus name: 

Storage conditions: 
Viral Storage Medium -80C
Sequencing: 
Complete genome
Infectivity: 
Infectivity tested and quantified
Mycoplasmic content: 
Mycoplasma free

Unit definition: 
vial containing 1 ml

In stock

1 500,00 €
(Cost per access for Academics)

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GMO: 
No
Biosafety restrictions: 
BSL1;
Virus host type: 
Previous Name or Taxonomy: 
Infectivity Test: 
Virus was heat-inactivated for 2 hours at 60C. Inactivation procedure was validated for other strains and has been shown e.g. here: doi: 10.1016/j.jobb.2020.12.001
Viral titer: 
dPCR E-gene (1.01E+08 cps/ml) ; RdRP (7.88E+07cps/ml)
Production cell line: 
Virus Cultivability: 
Passage: 
2
Technical recommendation: 
Following visual detection of cytopathic effect, virus culture supernatant was centrifuged for removal of cellular debris. Virus was heat-inactivated for 2 hours at 60C. Inactivation procedure was validated for other strains and has been shown e.g. here: doi: 10.1016/j.jobb.2020.12.001. The clarified supernatant was then aliquoted and frozen at -80C.
Shipping conditions: 
IATA Classification: 

Information about the collection of the virus

Recombinant product: 
No
Biological material origin: 
Natural origin
Collection date: 
Tuesday, 13 April, 2021
Country of collection: 
Isolation host: 
Isolation technique: 
Cell culture
Isolation conditions: 
5 days, 35°C and 5% CO2