SINDV-183-R2


EVAg

Produced by
: BMC-SAS
Shipping From
: Bratislava - SK

Product Description

Ref-SKU:
008v-EVA279
This was originally supposed to be isolated in 1975 from a frog Rana ridibunda (Kozuch et al., Acta virol. 22: 78, 1978), isolated and passaged on suckling mice and stored freeze-dried. This Sindbis virus[183-183-R2] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Product Risk Group: 
Virus name: 
Strain: 
Isolate: 

Storage conditions: 
Viral Storage Medium -80C
Sequencing: 
Partial sequence
Infectivity: 
Infectivity tested and quantified
Mycoplasmic content: 
Mycoplasma free

Unit definition: 
1 ml (2 vial of 0.5ml)

In stock

2 000,00 €
(Cost per access for Academics)

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GMO: 
No
Infectivity Test: 
Infectivity was proven by plaque titration. Determined virus titer was 1,5x 10^6 PFU/ml.
Viral titer: 
1500000 PFU/ml
Production cell line: 
Passage: 
Passaged 7 times on suckling mice in 1980 and stored freeze-dried. New stock prepared by one passage on Vero E6 cells on 8th december 2010.
Identification technique: 
RT-PCR followed by sequencing of the amplified product
Technical recommendation: 
We recommend to grow the virus on VeroE6 cells cultivated in MEM Eagle with Earle"s BSS (BioWhittaker, 12-136F). Following additives should be added to 500 ml of medium: 5ml Nonesential amino acids (Biochrom AG, K0293), 5 ml Sodium Pyruvate 100mM, (Biochrom AG, L0473), 5 ml L-Alanyl-L-glutamine 200mM (Biochrom AG, K0302), 5 ml Penicilin/ Streptomycin/ Amphotericin (BioWhittaker 17-745E), 25 ml FBS heat inact.s.American (Invitrogen, 10108-165). Multiplicity of infection (MOI) of 0,001 can be used for the infection and the infectious medium can be harvested 72hours after infection.