EVAg

Infectious Subgenomic Amplicons Reverse Genetic system for Zika virus strain Dak84 (derived from genbank sequence KU955592). It consists of three non infectious overlapping DNA fragments (cloned into pUC57 vector). These products enable to obtain infectious RNA virus after amplification by PCR, and transfection into cells,

Infectious Subgenomic Amplicons Reverse genetic system for Zika virus strain H/PF/2013. It consists of three non infectious overlapping DNA fragments (cloned into pUC57 vector). These products enable to obtain infectious RNA virus after amplification by PCR, and transfection into cells,

Infectious Subgenomic Amplicons Reverse genetic system for chimeric virus CH-17D / ZIKV. It consists of three non infectious overlapping DNA fragments (cloned into pUC57 vector) . These products enable to obtain infectious RNA virus after amplification by PCR, and transfection into susceptible cells. The chimeric virus systems were constructed using the yellow fever 17-D vaccine strain as a genetic backbone and by replacing prM/E from this vaccine strain with those of the Asian ZIKV PF epidemic strain.