EVA

The Public Health Laboratories of Hellenic Pasteur Institute (HPI) offer a comprehensive 5-day training course on Next-Generation Sequencing (NGS) for Influenza and Respiratory Syncytial Virus (RSV), using Oxford Nanopore Technology. This course is designed to provide participants with the knowledge and practical skills required to perform whole-genome sequencing and analysis of two major respiratory viruses—Influenza and RSV—supporting surveillance, research, and diagnostic development. Participants will have the opportunity to engage in real-life Influenza and RSV practises at the National Influenza Reference Laboratory of Southern Greece. Participants will gain both theoretical knowledge and hands-on experience in key NGS workflows, including: 1. Viral RNA extraction for NGS applications, 2. Library preparation for multiplexed sequencing, 3. Operation of Oxford Nanopore platforms, 4. Data Processing Workflow: Basecalling, Alignment, and Assembly, 5. NGS data quality control and validation, 6. Basic bioinformatics pipelines for Influenza and RSV virus genomics, 7. Bioinformatics tools for variant analysis and mutation detection. A special rate will be offered for accommodation in a 4-star hotel located near the Hellenic Pasteur Institute.