EVAg

cDNA of Zika virus isolate Brazil/2016/INMI1, obtained from reverse transcription with non specific hexamers primers. The cDNA was obtained from a Brasilian viral strain isolated in Italy, from a sample collected in January 28, 2016.

RNA of Human 2019-nCoV strain 2019-nCoV/Italy-INMI1. The RNA was obtained from a viral strain isolated in Italy, from a sample collected in January 29, 2020. To confirm its identity the virus has been completely sequenced. The complete sequence was submitted to GenBank (SARS-CoV-2/INMI1-Isolate/2020/Italy: MT066156) and is available on GISAID website (BetaCoV/Italy/INMI1-isl/2020: EPI_ISL_410545) upon registration. Import permit can be needed in your country. If yes, please indicate it in your online enquiry.

anti-Zaire ebolavirus (EBOV-Zaire) VP30

Rabbit polyclonal antiserum raised against BPL inactivated West Caucasian bat lyssavirus. The antiserum has been shown to specifically neutralize West Caucasian bat lyssavirus.

cDNA from West Caucasian bat lyssavirus. Prepared using random hexamers only or specific primer JW12. RNA extracted from virus cultured on BHK-21 cells using Trizol LS.

RNA from West Caucasian bat lyssavirus, RV3384, in HPLC water. The RNA was extracted from virus cultured in BHK-21 cell using Trizol LS.

Access to AMU/AFMB infrastructure for learning/training on techniques related to viral recombinant proteins

cDNA transcribed with random hexamers from total RNA extracted from Avian avulavirus 1, lineage 5b. This virus (APMV-1/pheasant/UK/739/2005) has been cultured in embryonated fowls eggs for 3 passages.

cDNA transcribed with random hexamers from total RNA extracted from Avian orthoavulavirus 1, lineage 4b. This isolate (APMV-1/partridge/UK(Scotland)/7575/2006) has been cultured in embronated fowls eggs for three passages.

infectious clone for the LR2006_OPY1 strain of Chikungunya virus, expressing GFP.

Training in Coronavirus reverse genetics to adapt novel techniques (ISA method) to large RNA viruses and compare different technologies under BSL-3 conditions

Lyophilized encapsidated RNA for SARS-CoV-2 real time RT-PCR targeting region of the N(CUB) gene (for 100 Rxns) AND Lyophilized ready-to-use primers and probe (Lyoph-P&P) for real time RT-PCR (96 rxns in the form of 4x24 rnxs). Target, primers and probe designed by Charité (CUB) Berlin. SOPs in the attached pdf. Disclaimer: This product does not contain the polymerase

Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 4x24 rxns) for real time RT-PCR of SARS-CoV-2 (2019-nCoV) in the N (CUB) gene. Primers and Probe sequences designed by Charité Universitätsmedizin Berlin; https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf). SOPs in the attached pdf. Disclaimer: This product does not contain the polymerase For RUO (research use only)

Lyophilized encapsidated RNA for SARS-CoV-2 (2019-nCoV) real time RT-PCR targeting region of the N (CUB) gene, for 100 Rxns (Target designed by Charité Universitätsmedizin Berlin). The encapsidated RNA has been stabilized to serve as positive control for extraction and real time RT-PCR. Extraction is mandatory before use in real time RT-PCR. SOPs in the attached pdf. For RUO (Research Use Only)

Lyophilized encapsidated RNA for SARS-CoV-2 (2019-nCoV) real time RT-PCR targeting region of the RdRp (IP2/IP4) gene, for 100 Rxns (Target designed by Institut Pasteur: https://www.who.int/docs/default-source/coronaviruse/real-time-rt-pcr-assays-for-the-detection-of-sars-cov-2-institut-pasteur-paris.pdf?sfvrsn=3662fcb6_2) The encapsidated RNA has been stabilized to serve as positive control for extraction and real time RT-PCR. Extraction is mandatory before use in real time RT-PCR. SOPs in the attached pdf. For RUO (Research Use Only)

Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 4x24 rxns) for real time RT-PCR of SARS-CoV-2 (2019-nCoV) in the RdRp (IP4) gene. Primers and Probe sequences designed by Institut Pasteur: https://www.who.int/docs/default-source/coronaviruse/real-time-rt-pcr-assays-for-the-detection-of-sars-cov-2-institut-pasteur-paris.pdf?sfvrsn=3662fcb6_2) SOPs in the attached pdf. Disclaimer: This product does not contain the polymerase For RUO (research use only)

Lyophilized encapsidated RNA for SARS-CoV-2 real time RT-PCR targeting region of the RdRp (IP2/IP4) gene (for 100 Rxns) AND Lyophilized ready-to-use primers and probe (Lyoph-P&P) for real time RT-PCR (96 rxns in the form of 4x24 rnxs). Target, primers and probe designed by Institut Pasteur Paris. SOPs in the attached pdf. Disclaimer: This product does not contain the polymerase

A bundle of rodent-borne virus stocks to be used for EQA preparation

Expression plasmids for the production of flaviviruses NS5 full length (MTase and RdRp domains). Synthetic gene optimized for E. coli. Coding sequence fused at its 5' with a cleavage 6His tag coding sequence

Hybridoma cell culture supernatant containing anti-BoHV 1 antibodies; targeted antigen: envelope; clone: 4H9; immunization antigen: recombinant envelope protein of BoHV 1 strain "Schönböken"

Bundle of different arenavirus RNA for various studies

The in vitro transcribed RNA is the control for the first-line screening assay termed E gene assay. The SARS-CoV full virus RNA is required to control the two confirmatory assays. Dislaimer: Import permit maybe be needed in your country for the SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry.

In vitro transcribed RNA for Wuhan coronavirus (2019-nCoV) real time (RT-)PCR targeting region of the E gene . Protocol and preliminary evaluation for this assays (Corman/Drosten 2020), as of Jan 13, 2020, available from: https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2 Ref: https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045

Expression plasmid for the Nterminal Domain of the MERS CoV Nucleocapsid coding sequence in fusion with a TRX/6-His tag encoding sequence at its 5'. For expression in E. coli

Recombinant expressed and purified receptor-binding domain (RBD) of SARS-CoV-2 for serological assays. The Protein was expressed in Expi293 cells and purified via Strep-Tactin XT and provided in Elution Buffer ( 100mM Tris-HCl; pH 8.0, 150mM NaCl, 1mM EDTA, 50mM Biotin).