EVAg

SARS-CoV-2 diagnostic training, including differential diagnostics of human Coronaviruses and other respiratory pathogens, incl. BSL-2 and BLS-3 conditions.

Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 4x24 rxns) for real time RT-PCR of SARS-CoV-2 (2019-nCoV) as of Jan 30, 2020, available from https://www.mdpi.com/1999-4915/12/2/159/pdf. Primers and Probe sequences designed by Charité Universitätsmedizin Berlin ; https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf. SOPs in the attached pdf. Disclaimer: This product does not contain the polymerase For RUO (research use only)

Lyophilized encapsidated RNA for SARS-CoV-2 (2019-nCoV) real time RT-PCR targeting region of the E gene, for 100 Rxns (Target designed by Charité Universitätsmedizin Berlin ; https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf). The encapsidated RNA has been stabilized to serve as positive control for extraction and real time RT-PCR. Extraction is mandatory before use in real time RT-PCR. SOPs in the attached pdf. For RUO (Research Use Only)

Lyophilized encapsidated RNA for 2019-nCoV real time RT-PCR targeting region of the E gene (for 100 Rxns) AND Lyophilized ready-to-use primers and probe (Lyoph-P&P) for real time RT-PCR (96 rxns in the form of 4x24 rnxs). Target, primers and probe designed by CUB Berlin. SOPs in the attached pdf. Disclaimer: This product does not contain the polymerase

RNA of Human 2019-nCoV strain 2019-nCoV/Italy-INMI1. To confirm its identity the virus has been completely sequenced. The complete sequence was submitted to GenBank (SARS-CoV-2/INMI1-Isolate/2020/Italy: MT066156) and is available on GISAID website (BetaCoV/Italy/INMI1-isl/2020: EPI_ISL_410545) upon registration. Import permit can be needed in your country. If yes, please indicate it in your online enquiry.

anti-GP 3 of Porcine reproductive and respiratory syndrome virus (PRRSV) murine monoclonal antibody, clone Hy P10-c40-c1 (CCLV-RIE 1167)

anti-N of Porcine reproductive and respiratory syndrome virus (PRRSV) murine monoclonal antibody, clone Hy P10-a1-10-c19 (CCLV-RIE 1168)

anti-GP 5 of Porcine reproductive and respiratory syndrome virus (PRRSV) strain I10, Hy P10-b38-c16 (CCLV-RIE 1089)

anti-GP 5 of Porcine reproductive and respiratory syndrome virus (PRRSV) strain I10 murine monoclonal antibody, clone Hy P10-a46-15 (CCLV-RIE 1175)

anti-GP 4 of Porcine reproductive and respiratory syndrome virus (PRRSV) murine monoclonal antibody, clone Hy P11.B2-b17 (CCLV-RIE 0375)

cDNA of Zika virus isolate Brazil/2016/INMI1, obtained from reverse transcription with non specific hexamers primers

Purified RNA of Coronavirus strain "BetaCoV/Germany/BavPat1/2020 p.1" grown in cell culture. Dislaimer: Import permit maybe be needed in your country. If yes, please indicate so in your online enquiry.

In vitro transcribed RNA for Wuhan coronavirus (2019-nCoV) real time (RT-)PCR targeting region of the E gene . Protocol and preliminary evaluation for this assays (Corman/Drosten 2020), as of Jan 13, 2020, available from: https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf

Purified RNA of SARS-Coronavirus strain Frankfurt 1, grown in cell culture. Dislaimer: Import permit maybe be needed in your country for the SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry.

Panel consists of (cell-culture) RNA from different coronaviruses (HCoV-Nl63, HCoV-229E, HCoV-OC43, MERS-CoV and SARS-CoV) and is intended for use as control of specificity of molecular assays. This panel contains sufficient RNA for t least 5 experiments. "Disclaimer: Import permit may be needed in your country for SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry". Product for Alphacoronavirus and Betacoronavirus. ------------------ NB: indicated price is only valid for non-profit laboratories. Commercial users will be contacted about pricing.

RG2 virus production (available on the EVAg catalogue) in single batch upon request containing 30 freeze-dried vials. The specific detection system (5 vials) by real-time PCR will be provided in the same package.

The in vitro transcribed RNA is the control for the first-line screening assay termed E gene assay. The SARS-CoV full virus RNA is required to control the two confirmatory assays. Dislaimer: Import permit maybe be needed in your country for the SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry.

Bundle of different arenavirus RNA for various studies

RNA extracted from infected cell culture supernatant of Bear Canyon virus, USA, 1998,

RNA extracted from infected cell culture supernatant of Oliveros Virus, Argentina, 1990

RNA extracted from infected cell culture supernatant of Latino virus

RNA extracted from infected cell culture supernatant of Cupixi virus, Brazil

RNA extracted from infected cell culture supernatant of Tamiami virus

RNA extracted from infected cell culture supernatant of Tacaribe virus

RNA extracted from infected cell culture supernatant of Parana virus, Paraguay, 1966