Plasmid for in vitro transcription that contains the 5`UTR region of an Aichi virus for 5`UTR-Leader region PCR assays published by Drexler et al, Emerg Infect Dis. 2011
In vitro transcribed RNA for Wuhan coronavirus (2019-nCoV) real time (RT-)PCR targeting region of the E gene .
Protocol and preliminary evaluation for this assays (Corman/Drosten 2020), as of Jan 13, 2020, available from:
https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2
Ref: https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045
In vitro transcribed RNA for Wuhan coronavirus (2019-nCoV) real time (RT-)PCR targeting region of the RdRP gene .
Protocol and preliminary evaluation for this assays (Corman/Drosten 2020), as of Jan 13, 2020, available from:
https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2
Ref: https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045
A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced and in vitro transcribed. In vitro transcript: Vector Sequence: Position 1-36, 727-816 Insert Sequence: Position 37-726
A specific genome region was amplified by PCR, cloned into a vector and in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 632-721 Insert Sequence: 37-631
A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced an in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 638-727 Insert Sequence: 37-637
A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced an in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 674-763 Insert Sequence: 37-673
A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced an in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 634-723 Insert Sequence: 37-633
In vitro transcribed RNA for new Betacoronavirus virus (hCoV-EMC) real time PCR targeting region upstream of the E gene (upE). Reference: Corman et. Al., Eurosurveillance, Volume 17, Issue 39, 27 September 2012
In vitro transcribed RNA for new Betacoronavirus virus (hCoV-EMC) real time PCR targeting region within the open reading frame 1a. Reference: Corman/Mueller et al., Eurosurveillance, Volume 17, Issue 49, 06 December 2012
The in vitro transcribed RNA is the control for the first-line screening assay termed E gene assay. The SARS-CoV full virus RNA is required to control the two confirmatory assays. Dislaimer: Import permit maybe be needed in your country for the SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry.