EVAg

in vitro transcribed RNA for Norovirus 2 real time PCR

Norovirus plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Target region of a specific real time assay was amplified and cloned.

Human herpesvirus 3 plasmid for a specific real time PCR.

in vitro transcribed RNA for human Astro virus real time PCR

Target region of a specific Real time assay was amplified and cloned.

Plasmid for in vitro transcription that contains the 5`UTR region of an Aichi virus for 5`UTR-Leader region PCR assays published by Drexler et al, Emerg Infect Dis. 2011

Human Coronavirus 229E plasmid for in vitro transcription.

in vitro transcribed RNA for Human coronavirus 229E real time PCR

In vitro transcribed RNA for Wuhan coronavirus (2019-nCoV) real time (RT-)PCR targeting region of the E gene . Protocol and preliminary evaluation for this assays (Corman/Drosten 2020), as of Jan 13, 2020, available from: https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2 Ref: https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045

In vitro transcribed RNA for Wuhan coronavirus (2019-nCoV) real time (RT-)PCR targeting region of the RdRP gene . Protocol and preliminary evaluation for this assays (Corman/Drosten 2020), as of Jan 13, 2020, available from: https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2 Ref: https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045

Extracted RNA of Human parainfluenzavirus 4.

in vitro transcribed RNA for Human coronavirus NL63 real time PCR

A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced and in vitro transcribed. In vitro transcript: Vector Sequence: Position 1-36, 727-816 Insert Sequence: Position 37-726

A specific genome region was amplified by PCR, cloned into a vector and in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 632-721 Insert Sequence: 37-631

A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced an in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 638-727 Insert Sequence: 37-637

A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced an in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 674-763 Insert Sequence: 37-673

A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced an in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 634-723 Insert Sequence: 37-633

In vitro transcribed RNA containing priming sites for different zika virus real time PCRs.

In vitro transcribed RNA for a specific SARS-coronavirus real time PCR.

In vitro transcribed RNA for new Betacoronavirus virus (hCoV-EMC) real time PCR targeting region upstream of the E gene (upE). Reference: Corman et. Al., Eurosurveillance, Volume 17, Issue 39, 27 September 2012

In vitro transcribed RNA for new Betacoronavirus virus (hCoV-EMC) real time PCR targeting region within the open reading frame 1a. Reference: Corman/Mueller et al., Eurosurveillance, Volume 17, Issue 49, 06 December 2012

The in vitro transcribed RNA is the control for the first-line screening assay termed E gene assay. The SARS-CoV full virus RNA is required to control the two confirmatory assays. Dislaimer: Import permit maybe be needed in your country for the SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry.

Internal control plasmid for in vitro transcription for HCV assays.

Hepatitis B Virus plasmid for realtime PCR (drosten et al.)